3,3'-[ethylenebis[(3-sulpho-p-phenylene)azo]]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonic acid] hexasodium salt AND 3,3'-[ethylenebis[(3-sulpho-p-phenylene)azo]]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonic] acid, compound with 2,2',2''-nitrilotriethanol (1:6)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

17 October 2005 to 05 December 2005

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study according to the guideline under GLP

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0 and 100 mg/L
- Sampling method: taken in duplicate at 0 (before daphnids were added) and 48 hours; test substance analyses: both duplicates (0 h), pooled sample in duplicate (48 h): controls single sample analysed in duplicate
- Sample storage conditions before analysis: stored at 4 °C and analysed after the 48 hours samples were taken

Vehicle:

no

Details on test solutions:

31.1 mg of the substance was dissolved in 300 mL water and stirred during 5 minutes at room temperature just before introduction of the daphnids..

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Strain: Daphnia magna Straus
- Source: inhouse bred
- Age at study initiation: < 24 hours
- Feeding during test: none

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

250 mg/L as CaCO3

Test temperature:

20-21 °C

pH:

7.6-8.0

Dissolved oxygen:

8.7-9.0 mg/L (96-101% of saturation)

Nominal and measured concentrations:

nominal: 0 and 100 mg/L
measured: ND and 98-99% of nominal

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL
- Type : closed with glass plate
- Material, size, headspace, fill volume: wide glass beaker containing 50 mL test solution
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water(according to guidance)
- Alkalinity: pH 7.8 ± 0.2
- Ca/Mg ratio: 4/1
- Na/K ratio: 10/1
- Culture medium different from test medium: similar

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16/8
- Light intensity: 570-740 Lux

EFFECT PARAMETERS MEASURED : immobilization

TEST CONCENTRATIONS
- Test concentrations: 0, 100 mg/L

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Test substance containing solutions were dark blue coloured.

Results with reference substance (positive control):

EC50 Potassium dichromate 0.53 mg/L

Reported statistics and error estimates:

NA

Validity criteria fulfilled:

yes

Conclusions:

The EC50 of the substance is > 100 mg/L

Executive summary:

In a test according to OECD 202 daphnids were exposed to 0 and 100 mg/L during 48 hours in a static test. No mortality or effects on mobility were found. The measured test concentrations were 98 -99% of nominal. The EC50 is > 100 mg/L.

Description of key information

In a test according to OECD 202 daphnids were exposed to 0 and 100 mg/L during 48 hours in a static test. No mortality or effects on mobility were found. The measured test concentrations were 98 -99% of nominal. The EC50 is > 100 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L