3,3'-[ethylenebis[(3-sulpho-p-phenylene)azo]]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonic acid] hexasodium salt AND 3,3'-[ethylenebis[(3-sulpho-p-phenylene)azo]]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonic] acid, compound with 2,2',2''-nitrilotriethanol (1:6)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10 August to 14 Augist 1987

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study on liquid formulation according to the guideline, but non-GLP. The tested concentration allows assessment of a reliable value for the LC50

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

no

Analytical monitoring:

yes

Details on sampling:

At all concentration at 0 and 96 hours
No information on sample storage conditions before analysis

Vehicle:

no

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: rainbow trout
- Strain:oncorhynchus mykiss
- Source: FORELLENHOF FREDELSLOH, DR.MED.VET. OTTO MUELLER D-3413 MORINGEN 1, Germany
- Age at study initiation (mean and range, SD): no data
- Length at study initiation: 5.1-6.8 cm (mean 5.9 cm)
- Weight at study initiation: 1.5-3.5 g (mean 2.4 g)
- Feeding during test: yes during maintenance ad libitum, food withheld 1 day (not indicated when, but most likely no feeding during the study and 24 hours before start)
- Food type: GROWING FEED F/B50, SSNIFF SPEZIALDIAETEN GMBH, Soest, Germany

ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions:same as test
- Type and amount of food: see above
- Health during acclimation: no mortality

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

2.5 MMOL/L

Test temperature:

12-13

pH:

7.3-8.0

Dissolved oxygen:

9.0-11.4 mg/L (82-104% of saturation)

Salinity:

NA

Nominal and measured concentrations:

nominal: 0, 500 and 1000 mg/L
measured: 0, 506.8 (t=0)/496.2 (t=96) mg/L, 1008.8(t=0)/992.3(t=96) mg/L

results based on nominal concentrations

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquarium (80CM X 35CM X 46CM) with 100 L water
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.24 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water
- acid capacity: 0.8 mmol/L
- Na/K: 10:1
- Ca/Mg ratio: 4:1

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light
- Light intensity: no data

EFFECT PARAMETERS MEASURED: at 0, 24, 48, 72 and 96 hours mortality/symptoms (due to extensive colour of the test solutions fish were directed to the front of the glass using a net)

TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: range finding test at 1000 mg/L did not show mortality
- Test concentrations: 0, 500 and 1000 mg/L

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 330 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Details on results:

no signs of toxicity were observed

Validity criteria fulfilled:

yes

Conclusions:

The LD50 of the substance is > 100 mg/L

Executive summary:

10 rainbow trouts/concentration were exposed to a formulation of the test substance at 0, 500 and 1000 mg/L. No mortaity or toxic effects were observed at any of the concentrations tested. Measured concentrations were within 80 -120% of nominal. It can be concluded that the LC50 of the active ingredient is >100 mg/L.

Description of key information

Ten rainbow trouts/concentration were exposed to a formulation of the test substance at 0, 500 and 1000 mg/L. No mortality or toxic effects were observed at any of the concentrations tested. Measured concentrations were within 80 -120% of nominal. It can be concluded that the LC50 of the active ingredient is >100 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L