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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
04 December 2015 - 25 July 2016
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
GLP compliance:
yes
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: B006
- Expiration date of the lot/batch: 24 May 2017
- Storage conditions: room temperature, dark, air-tight
- Storage area: desiccator
Vehicle:
yes
Test organisms (species):
Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Common name: Unicellular green algae
- Strain: ATCC22662
- Source (laboratory, culture collection): Americal Type Culture Colection

Algae have been mainteined by period subculture using Gorham meduim. Algae are kept under sterile condition. Sterile test has been performed periodically, every six months, to confirm that the aldae are not contaminated.
Test type:
static
Water media type:
freshwater
Total exposure duration:
72 h
Test temperature:
22 ± 2 °C
pH:
Not adjusted
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 0.07 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.07 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EL50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOELR
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Validity criteria fulfilled:
yes
Conclusions:
There were no significant circumstances that may have affected the reliability of the test results.
This test is valid because these results met the test validity criteria.

Results
The results given below were calculated from the time-weighted mean of measured concentration and the loading rate.

50% growth inhibition concentration, ErC5O (0-72h): >0.0702 mg/L (95% confidence limits: not available)
No observed effect concentration, NOECr (0-72h): 0.0702 mg/L

50% growth inhibition concentration, ErL5O (0-72h): >100 mg/L (95% confidence limits: not available)
No observed effect loading rate, NOELRr (0-72h): 100 mg/L

Description of key information

No observed effect concentration, NOECr (0-72h): 0.0702 mg/L

Key value for chemical safety assessment

EC10 or NOEC for freshwater algae:
0.07 mg/L

Additional information