oligomeric reaction product of 2-hydroxybenzaldehyde with 1-chloro-2,3-epoxypropane and phenol

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

07 October 2015 - 04 November 2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 835.3110 (Ready Biodegradability)

Test guideline 4

Qualifier:

according to guideline

Guideline:

other: Hungarian Good Laboratory Practice Regulations: 42/2014. (VIII. 19.) EMMI decree of the Ministry of Human Capacities which corresponds to the OECD GLP, 1997

Test guideline 5

Qualifier:

according to guideline

Guideline:

other: OECD Principles of Good Laboratory Practice, adopted by Council on 26th November 1997 [C(97)186/Final], Environment Directorate, Organisation for Economic Co-operation and Development, ENV/MC/CHEM(98)17, Paris 1998

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-({2-[bis({2-[(oxiran-2-yl)methoxy]phenyl})methyl]phenoxy}methyl)oxirane; 2-({2-[bis({4-[(oxiran-2-yl)methoxy]phenyl})methyl]phenoxy}methyl)oxirane; 2-{[2-({2-[(oxiran-2-yl)methoxy]phenyl}({4-[(oxiran-2-yl)methoxy]phenyl})methyl)phenoxy]methyl}oxirane

EC Number:

942-639-6

IUPAC Name:

2-({2-[bis({2-[(oxiran-2-yl)methoxy]phenyl})methyl]phenoxy}methyl)oxirane; 2-({2-[bis({4-[(oxiran-2-yl)methoxy]phenyl})methyl]phenoxy}methyl)oxirane; 2-{[2-({2-[(oxiran-2-yl)methoxy]phenyl}({4-[(oxiran-2-yl)methoxy]phenyl})methyl)phenoxy]methyl}oxirane

Test material form:

solid

Details on test material:

Name: EPICLON EXA-7250
Batch/Lot number: B006
Appearance: Brown solid (in smaller amount colour is yellow)
Purity: >99%
Manufacture date: 02 October 2014
Expiry date: 02 March 2017
Storage condition: Room temperature 15-25°C, below 70 RH%
Safety precautions: Routine safety precautions (lab coat, gloves, safety glasses, face
mask) for unknown materials were applied to assure personnel
health and safety.

Specific details on test material used for the study:

Name: EPICLON EXA-7250
Batch/Lot No.: B006
Description: Brown solid (in smaller amount the colour is yellow)
Purity: >99%
Molecular formula: C28H28O6 + (C19H18O4)n
Manufacture date: 02 October 2014
Expiry date: 02 March 2017
Storage condition: Controlled room temperature (15-25oC, below 70 RH%)
Safety precautions: Routine safety precautions (gloves, goggles, face mask, lab coat)
for unknown materials were applied to assure personnel health and
safety.

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

The sludge was washed by centrifugation, filtered through cotton wool and aerated until use.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

2.5 mg/L

Based on:

COD

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Results and discussion

% Degradation

% Degradation 1

Key result

Parameter:

% degradation (DOC removal)

Value:

6.65

Sampling time:

28 d

% Degradation 2

Key result

Parameter:

% degradation (O2 consumption)

Value:

> 0.16 - < 0.2

Sampling time:

28 d

BOD5 / COD results

BOD5 / COD

Key result

Parameter:

COD

Value:

7.4 other: mg O2/mg test mat.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

Under the test conditions the percentage biodegradation of EPICLON EXA-7250 reached a mean of 7.4 % after 28 days based on the measured COD of the test item. Therefore the test item is considered not readily biodegradable.
According to the test guidelines the pass level for ready biodegradability is removal of 60 % ThOD.
The reference item Sodium benzoate was sufficiently degraded to a mean of 74.2% after 14 days, and to a mean of 83.3 % after 28 days of incubation, based on ThODNH4, thus confirming the suitability of the used activated sludge inoculum.
In the toxicity control containing both, the test item and the reference item Sodium benzoate, a mean of 37.1 % biodegradation was noted within 14 days and 41.9% biodegradation after 28 days of incubation.
According to the test guidelines the test item can be assumed to be not inhibitory at the applied concentration level of 2.5 mg/L on the activated sludge microorganisms because degradation was >25 % within 14 days.