Registration Dossier

Toxicological information

Immunotoxicity

Currently viewing:

Administrative data

Endpoint:
immunotoxicity
Type of information:
experimental study
Adequacy of study:
other information

Data source

Referenceopen allclose all

Reference Type:
other: publication [Smialowicz1991a]
Title:
Unnamed
Year:
1991
Reference Type:
other: publication [Smialowicz1991b]
Title:
Immunotoxicity of 2-methoxyethanol following oral administration in Fischer 344 rats
Author:
Smialowicz RJ, Riddle MM, Luebke RW, Copeland CB, Andrews D, Rogers RR, Gray LE, Laskey JW
Year:
1991
Bibliographic source:
Toxicol Appl Pharmacol., 109: 494-506.

Materials and methods

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent

Results and discussion

Applicant's summary and conclusion

Executive summary:

F344 rats received 10 consecutive daily oral doses of Methoxyacetic acid ranging from 25 to 400 mg/kg bw in several experiments with somewhat diverging dosing regimens. At 100 and 200 mg/kg bw, thymic involution was observed in the absence of body weight reduction; there was also a reduction of lympho-proliferative responses to mitogens (Con A, PHA and PWM). At 200 mg/kg bw, the in vitro generated cytotoxic T-lymphocyte response was reduced, whereas mixed lymphocyte reaction and NKA were unaffected. The PFC response to TNP-LPS was suppressed throughout all dose levels, while increased to SRBC at 50 mg/kgbw. TNP-LPS- and SRBC-immunised rats dosed with Methoxyacetic acid showed suppression of PFC responses at 100 or 200 mg/kg bw and 200 or 400 mg/kg bw, respectively. Phenotypic analysis of splenocytes revealed a small (3%) reduction in the percentage of W3/25-positive cells (i.e. CD4, helper/inducer cells). Spleen cellularity appeared to be unaffected. Interleukin-2 production was decreased in the 150mg/kg bw group (Smialowicz et al, 1991a/b).