Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 268-640-5 | CAS number: 68132-47-8
Endpoint summary
Administrative data
Description of key information
Daphnia magna:Following exposure of the freshwater invertebrate, Daphnia magna to the test item, the EL50 (48h) value was reported as 3.7 mg/L loading rate WAF (confidence limits not determined). The No Observed Effect Loading Rate (NOEL) was reported as 1.0 mg/L loading rate WAF and the Lowest Observed Effect Loading Rate (LOEL) reported as 3.2 mg/L loading rate WAF (OECD 202 and EU Method C.2)
Algae: Exposure of Pseudokirchneriella subcapitata to the test item gave EL50 (72h) values of 26 mg/L loading rate WAF based on growth rate and 6.8 mg/L loading rate WAF based on yield. No Observed Effect Loading Rates (NOEL) were reported as 1.0 mg/L based on growth rate and yield (OECD 201 and EU Method C.3).
Additional information
Daphnia magna
A study was performed to assess the acute toxicity of the test item to Daphnia magna.The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (April 2004) No 202,"Daphnia sp., Acute Immobilisation Test" referenced as Method C.2 of Commission Regulation (EC) No. 440/2008.
Due to the low aqueous solubility and complex nature of the test item, for the purposes of the test, the test medium was prepared as a Water Accommodated Fraction (WAF). Following a preliminary range-finding test, and initial experiments, twenty daphnids (4 replicates of 5 animals) were exposed to Water Accommodated Fractions (WAFs) of the test item over a range of nominal loading rates of 1.0, 3.2, 10, 32 and 100 mg/L for 48 hours at a temperature of 21 to 22 °C under semi - static test conditions. The number of immobilised daphnia and any adverse reactions to exposure were recorded after 24 and 48 hours.
Chemical analysis of the fresh 1.0, 3.2, 10, 32 and 100 mg/L loading rate WAF test preparations at 0 hours showed measured test concentrations to range from 0.44 to 11 mg/L, and of the corresponding old or expired test preparations at 24 hours to range from 0.21 to 4.6 mg/L. Chemical analysis of the freshly prepared 1.0 and 3.2 mg/L loading rate WAF test preparations at 24 hours showed that measured concentrations of 0.29 and 0.80 mg/L were obtained respectively and of the corresponding old or expired test preparations at 48 hours showed that measured concentrations of 0.14 and 0.38 mg/L were obtained respectively. The dissolved test item may have been one or several components of the test item. Given that the toxicity cannot be attributed to a single component or a mixture of components, but to the test item as a whole, the results were based on nominal loading rates only.
Following exposure of the freshwater invertebrate, Daphnia magna to the test item, the EL50 (48h) value was reported as 3.7 mg/L loading rate WAF (confidence limits not determined). The No Observed Effect Loading Rate (NOEL) was reported as 1.0 mg/L loading rate WAF and the Lowest Observed Effect Loading Rate (LOEL) reported as 3.2 mg/L loading rate WAF.
Algae
A study was performed to assess the effect of the test item on the growth of the green alga Pseudokirchneriella subcapitata. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (2006) No 201, "Freshwater Alga and Cyanobacteria, Growth Inhibition Test" referenced as Method C.3 of Commission Regulation (EC) 761/2009.
Due to the low aqueous solubility and complex nature of the test item for the purposes of the test the test item was prepared as a Water Accommodated Fraction (WAF). Following preliminary range-finding tests, Pseudokirchneriella subcapitata was exposed to Water Accommodated Fractions (WAFs) of the test item over a range of nominal loading rates of 1.0, 3.2, 10, 32 and 100 mg/L (three replicate flasks per concentration) for 72 hours, under constant illumination and shaking at a temperature of 24 ± 1 °C. Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group, using a haemocytometer and light microscope.
Analysis of the test preparations at 0 hours showed measured test concentrations to range from 0.49 to 30 mg/L, A significant decline in measured test concentrations was observed at 72 hours in the range of less than the limit of quantification (LOQ) of the analytical method employed, determined to be 0.079 mg/L, to 0.23 mg/L. Given that the toxicity cannot be attributed to a single component or a mixture of components but to the test item as a whole, the results were based on nominal loading rates only.
Exposure of Pseudokirchneriella subcapitata to the test item gave EL50 (72h) values of 26 mg/L loading rate WAF based on growth rate and 6.8 mg/L loading rate WAF based on yield. No Observed Effect Loading Rates (NOEL) were reported as 1.0 mg/L based on growth rate and yield.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
