Reaction mass of 1-chlorodecane and 1-chlorotetraceane

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995-05-28 to 1995-11-07

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: 88/302/EWG

Version / remarks:

(1988)

Deviations:

no

Principles of method if other than guideline:

Method: other: Directive 88/302/EEC

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1-chlorotetradecane

EC Number:

219-368-0

EC Name:

1-chlorotetradecane

Cas Number:

2425-54-9

Molecular formula:

C14H29Cl

IUPAC Name:

1-chlorotetradecane

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

no

Details on preparation and application of test substrate:

The test substance was mixed with quartz sand before the mixture is added to the substrate. The substrate, the quartz sand with the test substance and water are mixed to obtain a homogenous substrate with a water content of approx. 40% (per dry weight). A control without test substance was also tested.

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name: earthworm
- Source: Eisenia GmbH, Wiesbaden. Germany
- Age at test initiation (mean and range, SD): 2 month
- Weight at test initiation (mean and range, SD): 300-600 mg, control mean 439 mg (303-565 mg), test substance mean 456 mg (338 - 599 mg)

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

yes

Total exposure duration:

14 d

Test conditions

Test temperature:

20 +/- 2 °C

pH:

6.6

Moisture:

40% water content in the substrate

Details on test conditions:

TEST SYSTEM
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
- Light intensity: approx. 400- 600 lux, for 24 h/day

SOURCE AND PROPERTIES OF SUBSTRATE (if soil): Soil substrate according to guideline 88/302/EWG

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality after 7 days and 14 days

TEST CONCENTRATIONS: 1000 mg test substance/kg soil dry weight

Nominal and measured concentrations:

1000 mg/kg soil dw

Reference substance (positive control):

yes

Remarks:

Chloroacetamide

Results and discussion

Effect concentrations

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

>= 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

No mortality (<10%) has been observed after 7 days and 14 days at a nominal test substance concentration of 1000 mg/kg soil dry weight.

Results with reference substance (positive control):

The toxicity of the reference substance has been tested at concentrations of 10, 30, 100 mg/kg. At 100 mg/kg no earthworm were found alive after 7 d and 14 d. The test is in the normal range and the test with the test substance is considered to be valid.

Any other information on results incl. tables

The highest test concentration of 1000 mg/kg did not lead to mortality after 7 and 14 days of exposure. (Validity criteria: <= 10 % mortality is  fulfilled.)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The toxicity of the test substance on Eisenia foetida has been examined in a limit test. The test substance concentration of 1000 mg/kg soil dry weight did not lead to mortality of the earthworms after 7 and 14 days of exposure.