Reaction mass of 1-chlorodecane and 1-chlorotetraceane

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995-11-03 to 1996-03-28

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: 92/69/EWG

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

Principles of method if other than guideline:

Method: other: Directive 92/69/EEC

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1-chlorotetradecane

EC Number:

219-368-0

EC Name:

1-chlorotetradecane

Cas Number:

2425-54-9

Molecular formula:

C14H29Cl

IUPAC Name:

1-chlorotetradecane

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

The test substance concentration was measured at test start and test end (72 h).

Test solutions

Vehicle:

no

Details on test solutions:

1 g/L of the test substance was dissolved in purfied water and stirred for 18 h. The solution was filtered and used as stock solution. The test substance concentration was analysed.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: green algae
- Strain: Chodat 86.81 SAG
- Source (laboratory, culture collection): Institute for hygiene of water, soil and air in Berlin, own cultivation
- Method of cultivation: A preculture was innoculated from a stock culture 3 days before test start.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Test temperature:

24.2 to 25°C

pH:

test start: 8.4 to 8.7, test end 9.0 to 9.3

Nominal and measured concentrations:

0.27 mg/L, 0.49 mg/L; 0.81 mg/L; 1.4 mg/L and 2.4 mg/L (test substance concentration determined for the stock solution before test start)

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer shake flasks, sterilized and aerated
- Initial cells density: 2*10^4 cells/mL
- Control end cells density: 1.09 *10^6 cells/mL
- No. of organisms per vessel: 2*10^4 cells/mL at 0 h
- No. of vessels per concentration (replicates): 5 replicates per test substance concentration
- No. of vessels per control (replicates): 8 replicates
- Standard medium used: as recommended in guideline

OTHER TEST CONDITIONS
- Sterile test conditions: sterillzed test vessels
- Adjustment of pH: no
- Light intensity: ~8000 lux

EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: photometric determination, absoprtion at 635 nm, measurement after 0 h, 24 h, 48 h, 72 h

TEST CONCENTRATIONS 0.27 mg/L, 0.49 mg/L; 0.81 mg/L; 1.4 mg/L and 2.4 mg/L

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

1.4 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

cell number

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

1.8 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

cell number

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 2.4 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

cell number

Effect concentrations 4

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 2.4 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other:

Remarks:

No inhibition observed at the highest test substance concentration.

Effect concentrations 5

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

> 2.4 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other:

Remarks:

No inhibition observed at the highest test substance concentration.

Details on results:

At the test substance concentration of 1.4 mg/L no inhibition on the growth (biomass) could be observed. The NOEC based on the biomass was determined to 1.4 mg/L (72 h). No inhibition on the growth rate has been observed at the highest test substance concentration (2.4 mg/L saturated solution). Therefore the effect concentration based on growth rate are above the highest tested test substance concentration.

Reported statistics and error estimates:

The inhibition rate has been calculated by comparison of the areas under growth curve.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

Within the highest concentration which could be obtained under the conditions of the test no effect could be observed at the highest concentration achieved under test conditions (2.4 mg/L) . Thus on the basis of cell growth, a mean effective concentration can be calculated to 72 h EbC50: > 2.4 mg/L (highest concentration under test conditions). On the basis of the growth rate, a mean effective concentration can be calculated to (0 - 72 h) ErC50: > 2.4 mg/L (highest concentration under test conditions). The NOEC value is 1.4 mg/L (based on cell growth). All concentrations are based on the substance.