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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1955
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Remarks:
Study was performed predating current guidelines

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1955
Report date:
1955

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Deviations:
yes
Remarks:
5 day observation period
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

1
Chemical structure
Reference substance name:
Reaction mass of 1-(2,6,6-trimethylcyclohex-2-en-1-yl)hepta-1,6-dien-3-one and 1-(2,6,6-trimethylcyclohex-1-en-1-yl)hepta-1,6-dien-3-one
EC Number:
904-551-6
Molecular formula:
C16H24O
IUPAC Name:
Reaction mass of 1-(2,6,6-trimethylcyclohex-2-en-1-yl)hepta-1,6-dien-3-one and 1-(2,6,6-trimethylcyclohex-1-en-1-yl)hepta-1,6-dien-3-one
Test material form:
liquid

Test animals

Species:
mouse
Strain:
CF-1
Sex:
not specified
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Carworth
- Housing: Individual cages
- Diet: Regular diet of Fox Blox

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
other: Distilled water plus Tween 20 to aid in mixing
Details on oral exposure:
VEHICLE
- Actual amount of test material fed: 0.1, 0.14, 0.16, 0.18, 0.20, 0.30, and 0.40 mL corresponding with 5, 7, 8, 9, 10, 15, and 20 mL/kg, respectively.
- Justification for choice of vehicle: distilled water and Tween 20 to aid mixing.

DOSAGE PREPARATION
The test material was diluted one part plus four parts with diluted water plus Tween 20.
Doses:
5, 7, 8, 9, 10, 15, and 20 mL/kg
No. of animals per sex per dose:
5, 5, 10, 10, 10,10, and 5 animals (sex unspecified) in the 5, 7, 8, 9, 10, 15, and 20 mL/kg dose goups, respectively.
Control animals:
no
Details on study design:
- Duration of observation period following administration: 5 days

Results and discussion

Effect levels
Key result
Sex:
not specified
Dose descriptor:
LD50
Effect level:
9 500 mL/kg bw
Based on:
test mat.
Remarks on result:
other: LD50 calculated with Behrens method
Mortality:
0/5, 0/5, 1/10, 4/10, 7/10, 9/10, and 5/5 animals died within the 5 day observation period after exposure to 5, 7, 8, 9, 10, 15, and 20 mL/kg, respectively.

Applicant's summary and conclusion

Interpretation of results:
other: Not classified
Remarks:
According to Regulation (EC) No. 1272/2008.
Conclusions:
The acute oral toxicity test showed an LD50 of 9500 mL/kg bw
Executive summary:

A preguideline study was performed to identify the acute oral toxicity of the test substance in mice. In this study, 5, 5, 10, 10, 10,10, and 5 mice (sex unspecified) were administered with 5, 7, 8, 9, 10, 15, and 20 mL/kg . substance at dose levels of 5, 7, 8, 9, 10, 15, and 20 mL/kg bw. 0/5, 0/5, 1/10, 4/10, 7/10, 9/10, and 5/5 animals died within the 5 day observation period after exposure to 5, 7, 8, 9, 10, 15, and 20 mL/kg, respectively. Under the conditions of the test, the acute oral LD50 for the substance in mice was determined to be 9500 mL/kg bw (calculated with Behrens method).