Sodium 4-[[4-[(4-hydroxy-2-methylphenyl)azo]phenyl]amino]-3-nitrobenzenesulphonate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

28 Apr - 11 May 1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Sodium 4-[[4-[(4-hydroxy-2-methylphenyl)azo]phenyl]amino]-3-nitrobenzenesulphonate

EC Number:

274-950-1

EC Name:

Sodium 4-[[4-[(4-hydroxy-2-methylphenyl)azo]phenyl]amino]-3-nitrobenzenesulphonate

Cas Number:

70865-20-2

Molecular formula:

C19H16N4O6S.Na

IUPAC Name:

sodium 4-[[4-[(4-hydroxy-2-methylphenyl)azo]phenyl]amino]-3-nitrobenzenesulphonate

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 100 mL and control
- Sampling method: 10 mL taken from approximate centre of test vessel at beginning of test
- Sample storage conditions before analysis: -20°C

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 1.5 g test item directly added to test vessel (volume 15L) and suspended
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): turbid suspension

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: rainbow trout
- Source: P Hohler, Zeinigen, Switzerland
- Length at study initiation (mean): 4.6 - 6.0 cm
- Weight at study initiation (mean): 1.5 g (control), 1.4 g (treatment)

ACCLIMATION
- Acclimation period: 7 d
- Acclimation conditions (same as test or not): tank 250 L in reconstituted water at 14.5 - 15.0°C with daily feeding
- Health during acclimation (any mortality observed): 0%

FEEDING DURING TEST
No

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

250 mg CaCO3 /L (dilution water)

Test temperature:

14.0 - 14.5°C

pH:

7.9 - 8.2

Dissolved oxygen:

8.9 - 11.6 mg/L

Nominal and measured concentrations:

100 mg/L (nominal)
70.5 mg/L (measured)

Details on test conditions:

TEST SYSTEM
- Test vessel: tank
- Material, size, headspace, fill volume: 15 L
- Aeration: yes
- No. of organisms per vessel: 10 (treatment), 7 (control)
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16h (light) / 8h (dark)
- Light intensity: 500 - 1500 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, clinical signs, size, body weight

TEST CONCENTRATIONS
- Spacing factor for test concentrations: limit test
- Range finding study
- Test concentrations: 0, 1.0, 10, 100 mg/L
- Results used to determine the conditions for the definitive study: yes, limit test in main study due to 0% mortality and no clinical signs in range finding study

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

>= 70.5 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 70.5 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: no
- Observations on body length and weight: no effect
- Other biological observations: no
- Mortality of control: 0%
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: turbid suspension and particles adsorbing to the vessel might have caused a mean recovery of ca. 70% after 96 h.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 96-h LC50 to fish is >70.5 mg/L (mean measured). No clinical signs of intoxication (sublethal effects) were observed during the test.