Registration Dossier

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
calculation (if not (Q)SAR)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
accepted calculation method
Justification for type of information:
Data is from computational model developed by USEPA
Qualifier:
according to
Guideline:
other: Modelling database
Principles of method if other than guideline:
Estimation 96 hrs LC50 value of test chemical on fish by EPI Suite ECOSAR version 1.11.
GLP compliance:
not specified
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
other: Fish
Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
2.336 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Neutral Organic SAR (Baseline Toxicity)
Validity criteria fulfilled:
not specified
Conclusions:
Based on effect of test chemical on the mortality of the test organism fish, the 96 hrs LC50 was estimated to be 2.336 mg/l.
Executive summary:

The short-term toxicity of the test chemical to fish is predicted using EPI Suite ECOSAR version 1.11. On the basis of effect of test chemical observed in a static freshwater system on the mortality of the test organism during the 96 hr exposure duration, the lethal effect concentration (LC50) for the test chemical is estimated to be 2.336 mg/l.Thus, based on the LC50 value, chemical can be considered as toxic to fish and thus can be considered to be classified in ‘aquatic chronic category 2’ as per CLP classification criteria.

Description of key information

The short-term toxicity of the test chemical to fish is predicted using EPI Suite ECOSAR version 1.11 (2018). On the basis of effect of test chemical observed in a static freshwater system on the mortality of the test organism during the 96 hr exposure duration, the lethal effect concentration (LC50) for the test chemical is estimated to be 2.336 mg/l.Thus, based on the LC50 value, chemical can be considered as toxic to fish and thus can be considered to be classified in ‘aquatic chronic category 2’ as per CLP classification criteria.

Key value for chemical safety assessment

LC50 for freshwater fish:
2.336 mg/L

Additional information

Predicted data of the test chemical and various supporting weight of evidence studies for its structurally similar read across chemical were reviewed for the short term toxicity to fish end point which are summarized as below:

 

In a prediction done using EPI Suite ECOSAR version 1.11, the short-term toxicity of the test chemical to fish is predicted. On the basis of effect of test chemical observed in a static freshwater system on the mortality of the test organism during the 96 hr exposure duration, the lethal effect concentration (LC50) for the test chemical is estimated to be 2.336 mg/l.

 

In a supporting weight of evidence study from authoritative database (J-CHECK, 2018),an acute toxicity test was conducted for 96 hrs for assessing the effect of test chemical on Oryzias latipes (Japanese rice fish). The test was performed in accordance to OECD guideline No. 203 “Fish Acute Toxicity Test”. Oryzias latipes (Japanese rice fish) was used as a test organism for the study. Test chemical conc. used for the study were1.00, 1.80, 3.20, 5.60 and 10.0 mg/l (geometric ratio ca. 1.8), control, vehicle control. Analytical monitoring involve the use of HPLC. Study was performed using 10 per conc. Oryzias latipes in a semi-static fresh water system. Total 50 fishes were exposed to test chemical in a vessel covered water surface with teflon sheet. The test vessels were placed under a photoperiod of room light, 16:8 hr light: dark conditions, respectively. On the basis of mortality of the test organism Oryzias latipes (Japanese rice fish), the 96 hrs LC50 value was determined to be 1.88 mg/l (95% C. I. – 1.42 to 2.48 mg/l), respectively. 

 

For the test chemical,short term toxicity to fish study was conducted for 96 hrs for assessing the effect of test chemical on fish. The study was performed under static conditions. On the basis of mortality of the test organism, the 96 hrs LC50 value was determined to be 1.8 mg/l. 

 

On the basis of the above results, it can be concluded that the test chemical can be considered astoxic to fish and thus can be considered to be classified in ‘aquatic chronic category 2’ as per CLP classification criteria.