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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
secondary literature
Justification for type of information:
Data is from secondary report.

Data source

Reference
Reference Type:
other: secondary source
Title:
SIDS Initial Assessment Report for 4-Methylbenzenesulfonyl chloride
Author:
United Nations Environmental Programme (UNEP)
Year:
2003
Bibliographic source:
SIDS Initial Assessment Report for 4-Methylbenzenesulfonyl chloride , United Nations Environmental Programme (UNEP) 2003

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
Principles of method if other than guideline:
To evaluate skin irritation potential of the given test chemical on new Zealand white rabbit.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
IUPAC name-4-Methylbenzenesulfonyl chloride
Molecular formula :C7H7ClO2S
Molecular weight :190.65 g/mol
Substance type: Organic
Physical state:solid
Smiles :S(=O)(=O)(c1ccc(cc1)C)Cl
InChI :1S/C7H7ClO2S/c1-6-2-4-7(5-3-6)11(8,9)10/h2-5H,1H3
Specific details on test material used for the study:
IUPAC name-4-Methylbenzenesulfonyl chloride
Molecular formula :C7H7ClO2S
Molecular weight :190.65 g/mol
Substance type: Organic
Physical state:solid
Smiles :S(=O)(=O)(c1ccc(cc1)C)Cl
InChI :1S/C7H7ClO2S/c1-6-2-4-7(5-3-6)11(8,9)10/h2-5H,1H3
Purity: 99.7%
Impurities:≤0.1%

Test animals

Species:
rabbit
Strain:
New Zealand White
Details on test animals and environmental conditions:
- Age at study initiation: 3-4 months old
-Number of animals: 3 animals
-Weight at study initiation: 2.1 - 2.3 kg
-Sex: Male animals

Test system

Type of coverage:
occlusive
Preparation of test site:
abraded
Vehicle:
not specified
Controls:
yes
Amount / concentration applied:
500mg in 0.1 ml sterile distilled water
Duration of treatment / exposure:
4 hrs
Observation period:
1, 24, 48 and 72 hours
Number of animals:
total = 3
Details on study design:
- Area of exposure: (2.5 x 2.5 cm)
-Type of wrap if used: three fold gauze patches
- Washing (if done): washed using warm water
- SCORING SYSTEM: Used (OECD method – Grading of skin reaction)

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
erythema score
Basis:
animal #3
Time point:
72 h
Score:
22
Remarks on result:
positive indication of irritation
Irritation parameter:
edema score
Basis:
animal #3
Time point:
72 h
Score:
15
Remarks on result:
positive indication of irritation
Irritant / corrosive response data:
erythema, scab and oedema were observed at the abraded and intact skins on the backs of three rabbits.

Any other information on results incl. tables

Change                                                        Erythema and Eschar

Applicated area

Intact

Abrarded

Phase (hrs)

1

24

48

72

1

24

48    

72

Animal ID (M01)

2

3

3

3

2

3

3

3

M02

2

3

3

3

2

3

3

3

M03

2

3

3

3

2

3

3

3

Total

6

9

9

9

6

9

9

9

Mean

2.0

3.0

3.0

2.0

3.0

3.0

3.0

3.0

Total of mean

                                                           22.0

Change                                                        Edema

Applicated area

Intact

Abrarded

Phase (hrs)

1

24

48

72

1

24

48    

72

Animal ID (M01)

1

2

2

2

2

2

2

2

M02

1

3

2

2

3

2

2

2

M03

1

2

2

1

2

2

2

1

Total

3

7

6

5

6

7

6

5

Mean

1.0

2.3

2.0

1.7

2.0

2.3

2.0

1.7

Total of mean

                                          15.0

Erythema and Eschar formation

0 – No erythema

1 – Very slight erythema (barely perceptible)

2 – Well defined erythema

3 – Moderate to severe erythema

4–Severe erythema (beef redness) to eschar formation preventing grading of erythema

- edema formation

0 – No oedema

1 – Very slight oedema (barely perceptible)

2 – Slight oedema (edges of area well defined by definite raising)

3 – Moderate oedema (raised approximately 1 mm)

4 – Severe oedema (raised more than 1 mm and extending beyond area of exposure)

 

Applicant's summary and conclusion

Interpretation of results:
Category 2 (irritant) based on GHS criteria
Conclusions:
The given test chemical was applied on the rabbit’s back in concentration 500 mg . Erythema, scab and edema were observed at the abraded and intact skins on the backs of three rabbits.Hence, it is considered to be moderately irritating
Executive summary:

The given test chemical was used to evaluate its skin irritation potential on New Zealand White rabbits. 500 mg of test material with about 0.1 mL of sterile distilled water was applied on abraded and intact sites on the shaved backs (2.5 x 2.5 cm) of rabbits. Left side of the back was treated as control. It was held in place for 4 hours with three fold gauze patches, which was applied in place with elasticity bandage then was fixed with non-irritating tape (3M paper-tape). After 4 hours, three fold gauze patches were removed and the exposed areas washed using warm water without altering the existing response or the integrity of the epidermis. The skin of animals was examined in accordance with an OECD scoring method for signs of erythema and oedema and the responses scored at 1, 24, 48 and 72 hours after the patches removal. Test chemical was applied on the rabbit’s back in concentration 500 mg. Erythema, scab and edema were observed at the abraded and intact skins on the backs of three rabbits. Hence, it is considered to be moderately irritating.