2,4,6-tri-n-propyl-2,4,6-trioxo-1,3,5,2,4,6-trioxatriphosphorinane

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

95-07-10 till 95-07-14

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

Adopted: Juli 17, 1992

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

Dated 07/31/1992

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Remarks:

Not possible, because the composition of the test water disturbs the analysis by paramagnetic effects

Vehicle:

no

Details on test solutions:

No auxiliary agent was used.

The substance was diluted in water, homogenized with the ultra-turrax and given into test vessels. Afterwards the dilution was stirred for 60 min and the pH was adjusted to a pH of 8 with 10 m NaOH.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Brachydanio rerio
- Strain: Hamilton-Buchanan
- Source: Corporate Toxicology (Hoechst AG)
- Date of hatching: 10/07/95
- Length at study initiation: 3.2 - 3.5 cm, mean 3.3 cm

ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions: same as test conditions
- Type and amount of food during acclimation: Tetra Min, Tetra Werke, Melle (FRG)
- Feeding frequency during acclimation: twice daily ad libitum

FEEDING DURING TEST
no feeding during test

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

2.1 - 2.5 mmol Ca2+ + Mg2+/L

Test temperature:

21 - 23 °C

pH:

7.6 - 8.1

Dissolved oxygen:

6.6 - 9.2 mg/L

Nominal and measured concentrations:

Nominal concentration: 0 and 100 mg/L
no measured concentrations

Details on test conditions:

TEST SYSTEM
- Test vessel: glass test chamber
- Material, size, fill volume: glass, length 30cm, width 22 cm, height 24 cm, 10 L volume
- Aeration: no
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: < 1 g fish/L water

TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: according to ISO/DIS 7346/1
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: In the 100 mg/L concentration vessel the pH was adjusted with 10 m NaOH
- Photoperiod: from 6 am to 6 pm
- Light intensity: 700 lux

Reference substance (positive control):

not required

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Observations on body length and weight: no
- Mortality of control: no
- Abnormal responses: no

Validity criteria fulfilled:

yes

Conclusions:

The test item was examined for acute toxicity to freshwater fish in a 96-h static test according to OECD Guideline 203 and EU method C.1. The test item caused no mortality up to a nominal concentration of 100 mg/L. Thus, the 96-h LC50 was determined to be greater than 100 mg/L.

Executive summary:

The acute toxicity of the test item to fish (zebrafish) was determined according to the OECD Guideline 203 and EU method C.1.

A static test was performed as a limit test with a concentration of 100 mg/L. Seven test organisms were exposed to the test concentration and the control, respectively. Mortality, visible abnormalities and changes in behaviour were determined after 24, 48, 72 and 96 hours. The test substance undergoes fast hydrolysis in water with subsequent temperature and pH-dependent changes in the composition of the hydrolysis products. Analytical determination of the test compound or of the hydrolysis products in the test batches was not possible, because the composition of the test water disturbs the analysis by paramagnetic effects.

Test organisms exposed to 100 mg/L of the test item showed no changes in behaviour and appearance. The test item caused no mortality up to a nominal concentration of 100 mg/L. Thus, the 96-h LC50 was determined to be greater than 100 mg/L.

Description of key information

The test item was examined for acute toxicity to freshwater fish in a 96-h static test according to OECD Guideline 203 and EU method C.1. The test item caused no mortality up to a nominal concentration of 100 mg/L. Thus, the 96-h LC50 was determined to be greater than 100 mg/L.

Additional information

The acute toxicity of the test item to fish (zebrafish) was determined according to the OECD Guideline 203 and EU method C.1.

A static test was performed as a limit test with a concentration of 100 mg/L. Seven test organisms were exposed to the test concentration and the control, respectively. Mortality, visible abnormalities and changes in behaviour were determined after 24, 48, 72 and 96 hours. The test substance undergoes fast hydrolysis in water with subsequent temperature and pH-dependent changes in the composition of the hydrolysis products. Analytical determination of the test compound or of the hydrolysis products in the test batches was not possible, because the composition of the test water disturbs the analysis by paramagnetic effects.

Test organisms exposed to 100 mg/L of the test item showed no changes in behaviour and appearance. The test item caused no mortality up to a nominal concentration of 100 mg/L. Thus, the 96-h LC50 was determined to be greater than 100 mg/L.