Bis(4-hydroxy-N-methylanilinium) sulphate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

Data is from secondary literature.

Qualifier:

according to guideline

Guideline:

other: As mentioned below

Principles of method if other than guideline:

Pimephales promelas Bioassay was carried out under static to study the effects of Bis(4-hydroxy-N-methylanilinium) sulphate on aquatic environment.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material: p-Methylaminophenol sulfate- IUPAC name: Bis(4-hydroxy-N-methylanilinium) sulphate- Molecular formula: C14H20N2O6S- Molecular weight: 344.386 g/mole- Smiles:CNc1ccc(cc1)O.CNc1ccc(cc1)O.OS(=O)(=O)O- Inchl: 1S/2C7H9NO.H2O4S/c2*1-8-6-2-4-7(9)5-3-6;1-5(2,3)4/h2*2-5,8-9H,1H3;(H2,1,2,3,4)- Substance type: Organic- Physical state: Solid crystalline (off white - white)

Analytical monitoring:

not specified

Vehicle:

yes

Remarks:

distilled water

Details on test solutions:

- Stock solutions: Stock solutions of the chemicals to be tested were prepared with distilled water. Appropriate volumes of these solutions were dispensed into the test containers, mixed with the diluent water.- Control : concurrent

Test organisms (species):

Pimephales promelas

Details on test organisms:

TEST ORGANISM- Common name: Fathead Minnow- Source: Muskie Hatchery, Asbury, New Jersey- Feeding during test: daily Feeding was suspended 2 days prior to each experiment.ACCLIMATION- Acclimation period: 2 weeks or longer- Acclimation conditions (same as test or not): same as test

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

54.5 (37.0 -75.0) F

pH:

7.0-7.5

Dissolved oxygen:

>=4 mg/l

Details on test conditions:

TEST SYSTEM- Test vessel: five 50-gallon glass aquaria- No. of organisms per vessel: 4 individuals/gallon- Aeration: 48-hour diffused aerationEach acclimation tank was equipped with three Metaframe Model118 "Bubble up" filter aerators. Each aerator consisted of achamber containing activated charcoal and cotton. A sourceof oil-free compressed air was supplied to each unit. Inaddition to providing continuous aeration and recirculation ofwater, these filter-aerators also served as a substrate forbiological growths, needed for the decomposition of fish wasteproducts.OTHER TEST CONDITIONSApplication frequency: 1X

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.25 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

coagulation of the embryo

Remarks on result:

other: toxic

Sublethal observations / clinical signs:

Bioassay data summary of Pimephales promelas

			Cumulative mortalities				Percent mortality at
Test chemical	Conc. (mg/l)	Starting population	24 hr	48 hr	72 hr	96 hr	96 hrs	96hr LC50 or range (mg/l)
4-methylAmino Phenol Sulfate (IIIP2)	0.25	15	8	0	0	0	53.3	0.25
	0.14	15	0	0	0	0	0.0
	0.08	15	0	0	0	0	0.0
	0.045	15	0	0	0	0	0.0
	0.025	15	0	0	0	0	0.0
	0.0	15	0	0	0	0	0.0

Conclusions:

The lethal concentration LC50 to 50 % of Pimephales promelas is 0.25 mg/l at 96 hrs. Mortality was observed during the assay. It can be concluded from the value that the Bis(4-hydroxy-N-methylanilinium) sulphate is toxic to the aquatic fish.

Executive summary:

A Pimephales promelas bioassay examined under static condition to study the effects of Bis(4-hydroxy-N-methylanilinium) sulphate at concentration range of 0.0, 0.025, 0.045, 0.08, 0.14, 0.25 mg/L.

Each bioassay series was conducted over the recommended 96 hour period. Moribund fish were removed from the test containers, and their numbers recorded on a daily basis. Daily monitoring for pH and dissolved oxygen was practiced. A minimum dissolved oxygen concentration of 4 mg/l was presumed acceptable for a valid experiment, and at no time during the program did the dissolved oxygen fall below this level.Similarly, no significant pH changes were observed. At least one control unit was included with every experiment. It contained the same number of fish as an individual test unit and served as a monitor of the diluent water. At highest concentration level, 53.3% mortality was observed in 96 hrs.

The lethal concentration LC50 to 50 % of Pimephales promelas is 0.25 mg/l. Hence from the value it can be concluded that the Bis(4-hydroxy-N-methylanilinium) sulphate is toxic to the aquatic fish and can be considered as “aquatic acute 1” as per classification for aquatic environment.

Description of key information

A Pimephales promelas bioassay examined under static condition to study the effects of Bis(4-hydroxy-N-methylanilinium) sulphate at concentration range of 0.0, 0.025, 0.045, 0.08, 0.14, 0.25 mg/L.

Each bioassay series was conducted over the recommended 96 hour period. Moribund fish were removed from the test containers, and their numbers recorded on a daily basis. Daily monitoring for pH and dissolved oxygen was practiced. A minimum dissolved oxygen concentration of 4 mg/l was presumed acceptable for a valid experiment, and at no time during the program did the dissolved oxygen fall below this level.Similarly, no significant pH changes were observed. At least one control unit was included with every experiment. It contained the same number of fish as an individual test unit and served as a monitor of the diluent water. At highest concentration level, 53.3% mortality was observed in 96 hrs.

The lethal concentration LC50 to 50 % of Pimephales promelas is 0.25 mg/l. Hence from the value it can be concluded that the Bis(4-hydroxy-N-methylanilinium) sulphate is toxic to the aquatic fish and can be considered as “aquatic acute 1” as per classification for aquatic environment.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.25 mg/L

Additional information

Following studies includes the experimental study for the target chemical and structurally similar read across to conclude the toxicity extent of 4-aminophenol (CAS: 55-55-0) towards fishe is summarized as follows:

The exoerimental study of the target chemical from Environmental Effect of Photoprocessing Chemicals Vol I and II 1974, suggests that, A Pimephales promelas bioassay examined under static condition to study the effects of Bis(4-hydroxy-N-methylanilinium) sulphate at concentration range of 0.0, 0.025, 0.045, 0.08, 0.14, 0.25 mg/L.

Each bioassay series was conducted over the recommended 96 hour period. Moribund fish were removed from the test containers, and their numbers recorded on a daily basis. Daily monitoring for pH and dissolved oxygen was practiced. A minimum dissolved oxygen concentration of 4 mg/l was presumed acceptable for a valid experiment, and at no time during the program did the dissolved oxygen fall below this level.Similarly, no significant pH changes were observed. At least one control unit was included with every experiment. It contained the same number of fish as an individual test unit and served as a monitor of the diluent water. At highest concentration level, 53.3% mortality was observed in 96 hrs.

The lethal concentration LC50 to 50 % of Pimephales promelas is 0.25 mg/l. Hence from the value it can be concluded that the Bis(4-hydroxy-N-methylanilinium) sulphate is toxic to the aquatic fish and can be considered as “aquatic acute 1” as per classification for aquatic environment.

Above study is supported by the another experimental study of target chemical from Environmental Protection Agency 1992, which also suggegsts that the Short-term toxicity to fish was carried out under static to study the effects of Bis(4-hydroxy-N-methylanilinium) sulphate on aquatic environment.

The lethal concentration LC50 to 50 % of Fathead minnows is 0.3 mg/l at 96 hrs. Mortality was observed during the assay. It can be concluded from the value that the Bis(4-hydroxy-N-methylanilinium) sulphate is toxic to the aquatic fish. Hence from the value it can be concluded that the Bis(4-hydroxy-N-methylanilinium) sulphate is toxic to the aquatic fish and can be considered as “aquatic acute 1” as per classification for aquatic environment.

Further the assissted by the structurally similar read across 4-aminophenol (CAS: 123-30-8) from J-Check 2010, inidctaes that the Short-term toxicity to fish test was carried out according to the OECD Guideline 203 (Fish, Acute Toxicity Test) at concentrations of2.00, 1.33, 0.889, 0.593, 0.395 mg/L to study the effects of 4-aminophenol on fish.

Test was carried out underflow-throughcondition at 24±1 degree C in 1.8 L per vessel, using 10 organismsper concentration. Aeration was not provided, Photoperiod: room light, 16 hours light - 8 hours dark. Mortalitywas observed during the test.

The lethal concentration LC50 to 50% ofOryzias latipesin 96 hr is 0.925 mg/L. It can be concluded from the value that the 4-aminophenol is toxic to the fish and can be considered as “Aquatic Acute 1” as per the classification for aquatic environment.

Thus based on the effect concentrations which is in the range 0.25 mg/l to 0.925 mg/l give the conclusion that test substance 4-aminophenol (CAS: 55-55-0) was likely to be toxic to fish at environmentally relevant concentrations and applying weight of evidence approach it can be considered to be“Aquatic Acute 1”as per the CLP classification criteria for aquatic environment.