Buta-1,2-diene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD guideline followed to GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

test volume increased and closed test vessels used to minimise loss of test substance by volatilisation

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

yes

Remarks:

test volume increased and closed test vessels used to minimise loss of test substance by volatilisation

GLP compliance:

yes (incl. QA statement)

Remarks:

Copy of certificate included

Test material

Test material information

Constituent 1

Reference substance name:

Buta-1,2-diene

EC Number:

209-674-2

EC Name:

Buta-1,2-diene

Cas Number:

590-19-2

Molecular formula:

C4H6

IUPAC Name:

buta-1,2-diene

Details on test material:

97.91% purity, manufactured in 1998 with a stability of >2 years

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
No data reported

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples taken at start and end of exposure period

Test solutions

Vehicle:

no

Details on test solutions:

For the prepartation of a stock solution, appr. 2ml of the test substance were mixed with 200ml deionised water and occasionally stirred during 18h, all at 4°C. This solution was diluted with deionised water at a temperature of 20°C. The resulting solution served as stock solution with a measured analytical value of 66.7mg TOC/l resp. 75.0mg substance/l. 5 test concentrations and a control.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

clone 5, bred in house, test organisms <24 hours old. "0 organissma in 4 parallel sets of 5 (±1) each

Study design

Test type:

other: Closed, static

Water media type:

freshwater

Total exposure duration:

48 h

Post exposure observation period:

None

Test conditions

Hardness:

250 mg/l CaCO3

Test temperature:

20°C ±1°C

pH:

7.7-8.2

Dissolved oxygen:

86-101 %

Salinity:

N/A

Nominal and measured concentrations:

nominal concentrations 3.3, 5.0, 6.7, 10.0 and 13.3mg.l-1. Geometric mean measured concentrations 2.8, 3.3, 4.5, 5.6 and 7.8mg.l-1

Details on test conditions:

To minimise the loss of the volatile test substance during the test, the volume inside the test vessels was increased to 20ml resulting in a reduced gas space. The vessels were closed tightly (gas tight septum).

Reference substance (positive control):

yes

Remarks:

potassium dichromate is tested as a reference substance quarterly

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

7.3 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 7.8 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Highest test concentration

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

5.6 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

As the nominal and measured concentrations deviated by more than 20% the effect concentration is reported as the geometric mean of the start and end concentrations.

Results with reference substance (positive control):

1mg K2Cr2O7 ≤50% effect.
2mg K2Cr2O7 ≥50% effect.

Reported statistics and error estimates:

No data reported

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

Fulfills validity criteria

Conclusions:

The reported 48hr EC50 with buta-1,2-diene is 7.3mg/l

Executive summary:

The reported 48hr EC50 with buta-1,2-diene is 7.3mg/l. This study is GLP compliant and follows a standard guideline. In addition, the data required to assess validity has been included.