3-hydroxy-3'-nitro-2-naphthanilide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1997

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-hydroxy-3'-nitro-2-naphthanilide

EC Number:

205-209-2

EC Name:

3-hydroxy-3'-nitro-2-naphthanilide

Cas Number:

135-65-9

Molecular formula:

C17H12N2O4

IUPAC Name:

3-hydroxy-N-(3-nitrophenyl)-2-naphthamide

Test material form:

solid

Details on test material:

purity: 99.7%
Product number HKAG 155

Specific details on test material used for the study:

purity: 99.7%

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The test substance was weighed into a beaker. Water for dilution was added and the mixture transferred quantitatively into a 5 liter glass bottle. The pH was adjusted with 10 molar NaOH to the pH of 10.0 to facilitate the dissolvation of the test substance in the test water. The chamber contents were stirred for approximately 24 hours with a magnetic-stirrer. Then the pH was adjusted to the pH of the test water with conc. HCI.
The suspension was filtered off with a cellulose filter to remove undissolved parts of the test substance.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish; Brachydanio rerio (Hamilton-Buchanan)
- Age at study initiation: approx. 5 months
- Length at study initiation: 2.5 - 3.0 cm
-Origin: Corporate Toxicology, Hoechst AG - Hoechst Marion Roussel

ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions: same as test
- Type and amount of food during acclimation: Tetra Min, twice a day ad libitum

FEEDING DURING TEST: no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

no

Test conditions

Hardness:

2.3 - 2.5 mmol Ca(2+) and Mg(2+)

Test temperature:

22 +-1 °C

pH:

7.9 - 8-1

Dissolved oxygen:

7.5 - 8.8 mg/L

Nominal and measured concentrations:

nominal : 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass vessel 20 cm x 15cm x 20 cm
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1


OTHER TEST CONDITIONS
- Photoperiod: 06.00 am to 06.00 pm
- Light intensity: approx. 700 Lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Test concentrations: 1000 mg/L nominal

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of this test the LC5O of Naphtol AS-BS after 96 hours was > 1000 mg/L (based on loading) and is above the solubility limit in the test water.

Executive summary:

Naphtol AS-BS was tested in zebra fish (Brachydanio rerio) over 96 hours in a static system. A limit test with a loading of 1 000 mg/L Naphtol AS-BS and a negative control (0 mg/L) was conducted.

After adding the substance the pH was adjusted with 10 molar NaOH to the pH of 10.0 to facilitate the dissolvation of the test substance in the test water. The chamber contents were stirred for approximately 24 hours with a magnetic-stirrer. Then the pH was adjusted to the pH of the test water with conc. HCI. The suspension was filtered off with a cellulose filter to remove undissolved parts of the test substance.

The tested concentration was present as light yellow solution. The test batch was homogeneous. No particulate matter was observed. The substance concentration was determined in the 1000 mg/L group. Due to the very low solubility of the test substance in water (water solubility = 0.35 mg/L), the values determined were in a range of only 0.60-0.68 mg/L (mean 0.64 mg/L). All lethality values given in this report are based on loading rates.

No lethality was observed up to the solubility limit of Naphtol AS-BS in the test water under the conditions of this test. The 1 000 mg/L group exhibited symptoms.