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EC number: 925-462-9 | CAS number: 1182723-73-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1998-09-07 till 1998-09-08
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study without restrictions.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: Commission Directive 88/302/EEC; Official Journal of the EC L 133 , Part C: Biodegradability: Test for inhibition of oxygen consumption
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- butan-1-aminium 3-[3-(4-fluorobenzenesulfonamido)-2,3,4,9-tetrahydro-1H-carbazol-9-yl]propanoate
- EC Number:
- 925-462-9
- Cas Number:
- 1182723-73-4
- Molecular formula:
- C25 H32 F N3 O4 S
- IUPAC Name:
- butan-1-aminium 3-[3-(4-fluorobenzenesulfonamido)-2,3,4,9-tetrahydro-1H-carbazol-9-yl]propanoate
- Reference substance name:
- 1-Butanaminium (R)-3-(fluorbenzenesulfonamido)-1,2,3,4-tetrahydro-9H-carbazole-9-prop
- IUPAC Name:
- 1-Butanaminium (R)-3-(fluorbenzenesulfonamido)-1,2,3,4-tetrahydro-9H-carbazole-9-prop
- Reference substance name:
- 1-Butanaminium (R)-3-(fluorbenzenesulfonamido)-1,2,3,4-tetrahydro-9H-carbazole-9-prop
- IUPAC Name:
- 1-Butanaminium (R)-3-(fluorbenzenesulfonamido)-1,2,3,4-tetrahydro-9H-carbazole-9-prop
- Test material form:
- other: solid
- Details on test material:
- - Purity: 58.3 %
- Empirical formula: C25H32FN3O4S
- Molecular mass: 489.2 g/mole
Constituent 1
Constituent 2
Constituent 3
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Direct weighings were prepared to give the different test item concentrations. The test item was added into Erlenmeyer flasks (incubation vessels) to about 130 mL deionised water and was stirred overnight.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - mixed population of different microorganisms, origin of an aeration tank of a waste water plant treating predominantly domestic sewage (Wupper
area water authority)
- pH: 6.8
- test concentration of the activated slugde: 480 mg/L
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- no data
- Test temperature:
- 18.6 - 20.9 °C (including all test concentrations, controls and reference substance)
- pH:
- 7.3 - 8.1 (including all test concentrations, controls and reference substance)
- Dissolved oxygen:
- Test substance: 6.0 - 9.5 mg O2/L at the beginning of the test and 2.5 - 8.8 mg O2/L at the end of the test.
Reference substance: 6.3 - 9.7 mg O2/L at the beginning of the test and 3.1 - 9.2 mg O2/L at the end of the test.
Control: 6.1 mg O2/L at the beginning of the test and 2.8 - 3.0 mg O2/L at the end of the test.
Physico-chemical of oxygen consumption control: 9.5 mg O2/L. - Nominal and measured concentrations:
- - nominal concentration of the test substance: 1000, 1800, 3200, 5600 and 10000 mg/L
- nominal concentration of the reference substance: 2.5, 5, 10, 20 and 40 mg/L - Details on test conditions:
- Test system:
- 300 mL glass Erlenmeyer flasks
- type of applcation: direct weighing
- test concentration of the actived sludge: 480 mg/L suspended solids
- temperature: 20 ± 2 °C
- stirring period before start of incubation: overnight
- two controls without test substance at test start and test end were performed in presence of activated sludge
- one physico-chemical oxygen consumption control was carried out at the highest test concentration od 10000 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol, purity: 97 % (Aldrich)
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4 950 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- EC50 of the reference substance 3,5-dichlorophenol is in the range 5 - 20 mg/L.
Inhibition: at 2.5 mg/L 12.8 %, at 5 mg/L 23.1 %, at 10 mg/L 50.0 %, at 20 % at 82.3 % and at 40 mg/L 89.0 %.
Any other information on results incl. tables
Results:
Test concentration | Respiratory rate | Phys. chem. | Respiratory rate | Inhibition |
test substance | O2 -cunsumption | phys. chem. O2 -consumption | ||
[mg/L] | [mg/L*h] | [mg/L*h] | [mg/L*h] | [%] |
1000 | 38.4 | 0.0 | 38.4 | 1.5 |
1800 | 35.0 | 0.0 | 35.0 | 10.3 |
3200 | 31.7 | 0.0 | 31.7 | 18.7 |
5600 | 16.3 | 0.0 | 16.3 | 58.2 |
10000 | 5.3 | 0.0 | 5.3 | 86.4 |
Control, mean | 39.0 | |||
Control 1 | 37.2 | |||
Control 2 | 40.8 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- (-respiratory rates of the two controls differ less than 15 %. - the EC50 of the reference compound 3,5-Dichlorophenol is in the range 5 - 20 mg/L.)
- Conclusions:
- The test item showed 18.7 % respiration inhibition of activated slugde at a test concentration of 3200 mg/L and 58.2 % of 5600 mg/L. An EC50 of 4950 mg/L was calculated. The effect value relates to nominal concentration; since no analytical monitoring was performed.
- Executive summary:
In order to test the toxicity to microorganisms of the substance, activated sludge from predominantly domestic sewage plant was exposed to the test solution of five nominal concentrations of the substance (1000, 1800, 3200, 5600 and 10000 mg/L), blank control solution and solution with positive reference substance for a period of 3 hours. Dissolved oxygen was recorded and respirationrates were calculated. No physico-chemical oxygen consumption was observed at the highest concentration of test substance.
The test item showed 18.7 % respiration inhibition of activated slugde at a test concentration of 3200 mg/L and 58.2 % of 5600 mg/L. An EC50 of 4950 mg/L was calculated. The effect value relates to nominal concentration; since no analytical monitoring was performed.
This toxicity study is classified as acceptable and satisfies the guideline requirements for the toxicity study to microorganisms.
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