Acylation product between cocoyl chloride and aminoacids

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2004-05-25 to 2004-08-31

Reliability:

2 (reliable with restrictions)

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

not specified

GLP compliance:

not specified

Analytical monitoring:

no

Details on sampling:

Not applicable

Vehicle:

no

Details on test solutions:

The mother solution preparation is carried out at a concentration of 100 mg/L.
The mother solution is diluted in order to obtain a concentration range that permit to calculate the EC50-72 hours; the tested concentrations are 10; 25; 50; 75; 100 mg/L

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

Species: Selenastrum capricornutum ATCC 22622
Source: Culture collection of Alage and Protozoa-Cumbria U.D., CCAP n°27814
In cultivation in the laboratoy.

Test type:

not specified

Water media type:

not specified

Limit test:

no

Total exposure duration:

72 h

Post exposure observation period:

No post exposure observation period

Hardness:

No data

Test temperature:

23°C +/- 2°C

pH:

No data

Dissolved oxygen:

No data

Salinity:

No data

Nominal and measured concentrations:

Nominal concentrations are 10; 25; 50; 75; 100 mg/L

Details on test conditions:

The test solution has to be prepared before adding algae.
At the same time that the control, we have to test concentrations forming a geometrical series that allows to obtain 100% growth inhibition after 72 hours and a series of intermediary inhibitions permitting to calculate EC50-72 hours.

Reference substance (positive control):

not specified

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

ca. 76 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Results with reference substance (positive control):

No data

Validity criteria fulfilled:

not specified

Conclusions:

After a 72 hours incubation, a growth inhibition of 50% of Selenastrum capricornutum appears for a 76 mg/L concentration.
EC50 72h Selenastrum capricornum = 76 mg/L

Executive summary:

Determination, in the conditions defined in this norm, of growth inhibition observed after 24, 48 and 72 hours.

EC50 -72 hours is obtained by calculation.

After a 72 hours incubation, a growth inhibition of 50% of Selenastrum capricornutum appears for a 76 mg/L concentration. EC50 72h Selenastrum capricornum = 76 mg/L

Key value for chemical safety assessment

EC50 for freshwater algae:

76 mg/L