Lithium 4-[(5-amino-3-methyl-1-phenyl-1H-pyrazol-4-yl)azo]-2,5-dichlorobenzenesulphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

other: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Justification for type of information:

Justification for Read Across is reported in the endpoint summary and it is further detailed in the report attached to the IUCLID section 13.

Principles of method if other than guideline:

Internal method Nr. 11.
The acute fish toxicity test was performed according to published methods, which were adapted and standardized for the specific needs of the company, well before Test Guidelines for these methods were established by the OECD beginning 1981. These methods were comparable to current standard testing procedure
published by the OECD within Guideline for Testing of Chemicals 203: Fish, Acute Toxicity Test, adopted by the Council on 17th July 1992.

GLP compliance:

no

Remarks:

pre GLP

Test organisms (species):

Salmo sp.

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout.
- Supplier: P. Hohler, 4314 Zeiningen.
- Age at study initiation: about 4 - 6 months.
- Length at study initiation: 4 - 8 cm
- Weight at study initiation: 3 - 6 g
- Feed: daily (TETRA MIN, flocks, Dr. U.Baersch GmbH, D-4520 Melle, Germany).

ACCLIMATION
- Acclimation period: minimum 12 days.
- Acclimation conditions: temperature of 10-13 °C.

Water media type:

freshwater

Total exposure duration:

48 h

Test temperature:

12 °C

pH:

8 - 8.2

Dissolved oxygen:

O2-concentration was be kept at 4.8 mg/l at a minimum. Sufficient oxygen supply was ensured. O2-target concentration 6.4 mg/l

Nominal and measured concentrations:

Nominal concentrations: 100, 200, 1000 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: full-glass basins, 17 liters basis (36x23x26 cm) tempered (cold-room), filled by 15 liters.
- No. of organisms per concentration: 8 per test concentration.

TEST MEDIUM / WATER PARAMETERS
- Dilution water: water used according to ISO guidelines.
- Preparation: 7.5 ml of each one of the stock-solutions 1 and 2 and 75 ml of stock-solution 3, add 15 liter of deionized water.
Stock-solution 1: 250 g CaCl x 2 H2O, 29 g NaCl, 9 g NaNO3, add 1 liter of deionized water
Stock-solution 2: 115 g MgSO x 7 H2O, 79 g Na2SO4, add 1 liter of deionized water.
Stock-solution 3: 27 g NaHO3, add 1 liter of deionized water.

Duration:

48 h

Dose descriptor:

other: TL50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No deaths occurred.

Sublethal observations / clinical signs:

Conc. (mg/l)	Survivors		pH at 48 hrs
	24hrs	48hrs
100	8	8	8.1
200	8	8	8.2
1000	8	8	8

Conclusions:

TL50 (48h) > 1000 mg/l (nominal)

Executive summary:

Method

The acute fish toxicity test was performed according to published methods, which were adapted and standardized for the specific needs of the company, well before Test Guidelines for these methods were established by the OECD beginning 1981. These methods were comparable to current standard testing procedure published by the OECD within Guideline for Testing of Chemicals 203: Fish, Acute Toxicity Test, adopted by the Council on 17th July 1992. Eight trouts per concentration were exposed at 100, 200 and 1000 mg test item/l, over a period of 48 hours.

Results

At the end of the experiment, no death was recorded, thus TLC50 (48h) resulted to be higher than 1000 (nominal).

Description of key information

Non harmful/toxic to fish, short-term exposure

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

613 mg/L