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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
other: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
1994
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions
Remarks:
Experimental study performed on the non-salified form of the substance under registration. The difference in counter-ion does not impact the study outcomes because the ecotoxicological properties are determined by the base of the substance.
Qualifier:
according to guideline
Guideline:
ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
Deviations:
not specified
GLP compliance:
no
Analytical monitoring:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia Magna.
- Strain: Straus.
- Source: own laboratory breeding.
- Age at study initiation: young daphnia born in 24 hours.
- Method of breeding: aperiodic parthenogenesis. The grow-up gravid females are separated into dilution water.
- Feeding during test: no.

ACCLIMATION
- Acclimation period: young daphnia born in 24 hours are segregated to the handling container from which they entered the experiment.

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
20 ± 2 °C
pH:
7.8 ± 0.2
Dissolved oxygen:
> 7 mg/l
Nominal and measured concentrations:
Nominal: 100, 800, 700, 600, 500, 400, 200, 100, 60 mg/l
Details on test conditions:
TEST SYSTEM
- Biomass loading rate: 5 Daphnia in 10 ml solution.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: the dilution water was prepared just before the test by admixing 25 ml of stored solutions A, B, C and D into 1 000 ml of distilled water.
A. 11.76 g CaCl2.2H2O in 1 litre of distilled water.
B. 4.93 g MgSO4.7H2O in 1 litre of distilled water.
C. 2.59 g NaHCO3 in 1 litre of distilled water.
D. 0.23 g KCl in 1 litre of distilled water.
- Hardness: CaCO3 250 ± 25 mg/l.
- Conductivity:0.1 µS/m
- Ratio Ca:Mg: 4 : 1
Reference substance (positive control):
yes
Remarks:
the sensitivity of the test species and correctness of test performance is periodically verified in three-month period by testing with the refence substance, potassium dichromate.
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
302 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Details on results:
EC50 (24h): 608 mg/l (nominal)
Validity criteria fulfilled:
yes
Remarks:
immobilisation in the controls did not exceed 10 % at the end of the test and the dissolved oxygen concentrations (throughout the test) remained above 2 mg/l
Conclusions:
EC50 (48h): 302 mg/l (nominal)
Executive summary:

Method

The substance was tested on Acute immobilisation test on Daphnia magna, according to ISO 6341 15 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea)). Daphnids were exposed to the nominal concentration of 100, 800, 700, 600, 500, 400, 200, 100, 60  mg/l, over a period of 48 hours.

Results

EC50 (24h): 608 mg/l (nominal)

EC50 (48h): 302 mg/l (nominal)

Description of key information

Non harmful/toxic to aquatic invertebrates, short-term exposure

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
302 mg/L

Additional information