3,3,4,4,5,5,6,6,7,7,8,8-dodecafluorodeca-1,9-diene

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From December 2014 to April 2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Deviations:

yes

Remarks:

minor deviation which has no impact on the quality and the integrety of the study

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.6200 (Earthworm Subchronic Toxicity Test)

Deviations:

yes

Remarks:

minor deviation which has no impact on the quality and the integrety of the study

Qualifier:

according to guideline

Guideline:

other: MEP, P.R. China the Guidelines for the Testing of Chemical No. 207 "Earthworm, Acute Toxicity Test" (Version 2, August 2013).

Deviations:

yes

Remarks:

minor deviation which has no impact on the quality and the integrety of the study

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

- Appearance: Clear colourless liquid
- Test item storage: At room temperature protected from light
- Purity/Composition correction factor: No correction factor required

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Eisenia fetida

Animal group:

nematods

Details on test organisms:

The test earthworm (Eisenia foetida) were obtained from Chenggong Earthworm Raising Farm where in Dongli District, Tianjin.
Earthworms had been conditioned for 24 hours in an artificial soil, washed quickly and dried on filter paper. Earthworms were weighed before use and placed on the surface of test medium. Worms were adults (more than two months old with clitellum) with an individual weight of 318 to 593 mg.

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

yes

Total exposure duration:

14 d

Test temperature:

18.18 -21-98°C

Moisture:

65-82%

Details on test conditions:

TEST SYSTEM
- Test container (material, size): height: 19cm X length: 12 cm X width: 9 cm. The containers were covered with a glass cover to prevent the earthworms from escaping and drying of the test medium.
- Amount of substrate: 750 g wet weight
- No. of replicates per treatment group: 4 (10 earthworms per replicate)
- No. of replicates per control: 4 (10 earthworms per replicate)



OTHER TEST CONDITIONS
- Photoperiod: continuous illumination
- Light intensity: 411 to 555 lux

EFFECT PARAMETERS MEASURED:
- Survival: Observations were conducted on day 7 and 14. Mortality was assessed by emptying the test medium onto a plate, sorting worms from the medium and testing their reaction to a mechanical stimulus at the front end. After the day 7 assessment, worms and medium were returned to the test containers. Observed behavioral or pathological symptoms were recorded.
- Weight: At the end of the test, the individual weights of all surviving worms in each group were recorded; and mean body weights were calculated for each treatment group as well as the control.


RANGE FINDING TEST CONCENTRATIONS
- Range finding study: Limit test
- Test concentrations: 1000 1000 mg/kg dry soil weight.
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

yes

Remarks:

chloracetamide

Key result

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

7 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

>= 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

MORTALITY AND TOXIC SIGNS:
During the exposure period, no deaths and toxic signs were observed in control group and 1000 mg/kg dry soil weight test group.

WEIGHT:
The earthworm mean weight of the control and test group were 0.444 g and 0.456 g, respectively, at beginning of the test.
The weight was slightly decrease at the end of the test, the mean weight were 0.388 g and 0.416 g respectively and decrease rate were 12.6% and 8.8% for the control and test group.

Results with reference substance (positive control):

The LC50 (14 day) result of the reference substance to earthworm is 24.4 mg/kg dry soil weight, with 95% confidence limits of 22.5-26.5 mg/kg dry soil weight.

Reported statistics and error estimates:

According to the results, no statistical method was used.

Validity criteria fulfilled:

yes

Conclusions:

Under the test conditions, the 7-day LC50 and the 14-day LC50 was greater than 1000 mg/kg dry soil weight.

Executive summary:

The acute toxicity of the test item to Eisenia fetida was investigated in a GLP-compliant study performed in accordance with OECD Guideline No. 207. Under the test conditions, the 7-day LC50 and the 14-day LC50 was greater than 1000 mg/kg dry soil weight.

Description of key information

The acute toxicity of the test item to the earthworm Eisenia fetida was investigated in a GLP-compliant study performed in accordance with OECD Guideline No. 207. Under the test conditions, the 7-day LC50 and the 14-day LC50 was greater than 1000 mg/kg dry soil weight.

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for soil macroorganisms:

1 000 mg/kg soil dw

Additional information

The acute toxicity of the test item to Eisenia fetida was investigated in a GLP-compliant study performedin accordance with standard methods, with one minor deviation. The study is considered as reliable (Klimisch 1) and is selected as a key study for the endpoint.