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Effects on developmental toxicity

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Type:Developmental or Reproductive Toxicity

Species: pregnant rats BD

Doses: single iv injection of 20 mg DMS/kg body weight

Result: Among 59 offspring, observed for more than 1 year, 7 developed malignant tumors, including 3 tumors of the brain (at 466, 732, and 907 days). Other tumors included 1 adenoma of the thyroid, 2 hepatic-cell carcinomas, and 1 carcinoma of the uterus.
Reference: IPCS INCHEM; Environmental Health Criteria (EHC) Monographs. Dimethyl sulfate (EHC 48, 1985). Available from, as ofOctober 1, 2007:


Type: Developmental or Reproductive Toxicity

Species: pregnant Crl:CD BR rats

Route of exposure: inhalation (nose-only)

Result: Rats were exposed to 0.1, 0.7 or 1.5ppm dimethyl sulfate (DMS) for 6 hr/day from Days 7 through 16 of gestation (day in which copulation plug was detected was designated Day 1G). A control group of pregnant rats was exposed simultaneously to air only. All female rats were euthanized on Day 22G and the fetuses were examined. A suppression of both food consumption and the rate of body weight gain were seen in the 0.7 and 1.5 ppm groups. No unusual clinical signs were seen in rats exposed to DMS. None of the reproductive parameters was altered in any of the groups and no statistically significant fetal effects were detected. DMS is not a developmental toxicant in the rat following inhalation exposures up to 1.5 ppm during the period of major organogenesis.
Reference: Alvarez et al; Drug Chem Toxicol 20 (1-2): 99-114 (1997)PubMed Abstract

Type: Developmental or Reproductive Toxicity

Result: In mice and rats, inhalation ofdimethyl sulfateat 0.1-4 ppm throughout pregnancy caused preimplantation losses and embryotoxic effects, including anomalies of the cardiovascular system.
Reference: American Conference of Governmental Industrial Hygienists, Inc. Documentation of the Threshold Limit Values and Biological Exposure Indices. 6th ed. Volumes I, II, III.Cincinnati,OH: ACGIH, 1991, p. 497.

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