Registration Dossier

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Experiment start and end date - 16 October 1990; Study completion date - 11 December 1990.
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
no
Qualifier:
according to guideline
Guideline:
ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
Deviations:
no
Qualifier:
according to guideline
Guideline:
other: ETAD-Method No.103
GLP compliance:
yes
Specific details on test material used for the study:
Test substance: FAT 20004/H
Storage: room temperature
Appearance: Solid
Purity: 100 %
Analytical monitoring:
no
Vehicle:
not specified
Test organisms (species):
activated sludge
Details on inoculum:
mixed culture of bacteria (sludge of a Husman apparatus)
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Test temperature:
19.5 °C
Nominal and measured concentrations:
1, 3.2, 10, 32, 56 and 100 mg/L
Details on test conditions:
O2 consumption, measured with an oxygen-sensitive electrode system: OXI DIGI 530.
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol
Duration:
3 h
Dose descriptor:
IC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
The IC50 of the reference substance 3,5-Dichlorophenol is: 18 mg/l (Criteria: 10 - 25 mg/l)
Validity criteria fulfilled:
yes
Conclusions:
The IC50 (3 h) of the test substance FAT 20004/H was found to be greater than 100 mg/l.
Executive summary:

The objective of this study was the determination of the test substance concentration, which reduces the respiration of activated sludge by 50 % (IC50 value). The toxicity of FAT 20004/H to activated sludge was determined in a 3 hours respiration inhibition test according to the OECD Guideline No. 209, and to the EG Method Nr.L 133/118. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland. The test substance concentrations tested were 1, 3.2, 10, 32, 56 and 100mg/l. The reference substance used was 3, 5-Dichlorophenol in concentrations of 32, 10 and 3.2 mg/l.


Results:


IC50 (3 h) of the test substance FAT 20004/H: >100.0 mg/l


IC50 (3 h) of the reference substance 3, 5-Dichlorophenol: 18 mg/l

Description of key information

The IC50 of FAT 20004 was found to be >100 mg/l.

Key value for chemical safety assessment

EC50 for microorganisms:
100 mg/L

Additional information

In a key study carried out to determine IC50 of FAT 20004/H according to GLP guideline 209, the IC50 value for FAT 20004 was found to be greater than 100 mg/L. Several other supporting studies were also performed and the IC50 value for FAT 20004 was found to be greater than 300 & 200 mg/L.