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Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: inherent biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Experiment start date - 09 October 1990; Experiment end date - 14 November 1990; Study completion date - 14 November 1991.
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
Deviations:
yes
Remarks:
Sludge concentration: 1000 mg/l
Qualifier:
according to guideline
Guideline:
other: EEC Directive, L133 (pp.99-105) Volume 31, May 88 (ISSN 0378-6978)
Deviations:
yes
Remarks:
Sludge concentration: 1000 mg/l
Qualifier:
according to guideline
Guideline:
other: ISO Method 8192 B
Deviations:
yes
Remarks:
Sludge concentration: 1000 mg/l
GLP compliance:
yes
Specific details on test material used for the study:
Name: FAT 20004/H
Storage: room temperature
Appearance: solid
Oxygen conditions:
not specified
Inoculum or test system:
other: Sludge of a domestic sewage treatment plant
Details on inoculum:
Sludge of a domestic sewage treatment plant (ARA-Rhein) collected on 16.10.90
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
Test concentration: 100 mg/l DOC nominal
Water / Nutrient According to the specification of the EEC L133
(pp.99-105) Volume 31 resp. OECD Method 302B.
Temperature: 22 ± 3 °C
Duration: 28 days
Lighting: Indirect daylight
Estimation technique: TOC/DOC SHIMADZU TOC-500
Reference substance:
diethylene glycol
Remarks:
Fluka Nr. 32170
Parameter:
% degradation (DOC removal)
Value:
97.75
Sampling time:
28 d
Remarks on result:
other: Adsorption after 3 hours: 54.3 %
Details on results:
Elimination measured as DOC (mg/l) after 28 days corrected by the blank control is: 97.75 % (average of 2 tests running in parallel)
Adsorption after 3 hours: 54.3 %
Results with reference substance:
Biodegradation corrected by the blank control and measured as DOC (mg/1) is: 94.8 % (28th day).

Experiment 1:

































































Time Elimination (%)
0 hour61.9
3 hours53
2 days53.6
5 days91.3
7 days87.8
9 days90.3
12 days93.5
14 days91.5
16 days94.2
19 days100
21 days100
23 days100
26 days100
28 days100

 


Experiment 2:

































































Time Elimination (%)
0 hour66.2
3 hours55.6
2 days86.9
5 days90
7 days86.6
9 days87.7
12 days91.5
14 days88.6
16 days95
19 days100
21 days100
23 days100
26 days100
28 days100
Validity criteria fulfilled:
yes
Interpretation of results:
inherently biodegradable
Conclusions:
The biodegradation of the test substance FAT 20004/H after 28 days was 97.75 %
Executive summary:

The inherent biodegradability of FAT 20004/H was determined in a Zahn-Wellens / EMPA ­Test according to the OECD Guideline for Testing of Chemicals, No. 302B, 1992. The reference substance used was Diethylene glycol in the test. 


Biodegradation:


The biodegradation of the test substance FAT 20004/H after 28 days was: 97.75 %


Total Elimination: (regarding the adsorption and the biodegradation)


The total elimination of the test substance FAT 20004/H after 28 days was: 97.75 % (average of 2 tests running in parallel)

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Experiment start date - 01 November 1990; Experiment end date - 11 November 1990; Study completion date - 04 February 1991.
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
other: EEC, Annex V, Part. C.8, corresponding to DEV H-5 (a2), Ausgabe 1966
GLP compliance:
yes
Specific details on test material used for the study:
Test substance: FAT 20004/H
Storage: room temperature
Appearance: solid
Oxygen conditions:
not specified
Inoculum or test system:
other: mixed culture of bacteria (effluent of a Husman laboratory apparatus)
Duration of test (contact time):
2 h
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
Experimental conditions
Test concentration - 1, 5, 10, 20, 50, 100, 200 and 500 mg/l
Temperature - 22 ± 2 °C
Duration - 5 days
Reference substance:
benzoic acid, sodium salt
Parameter:
other: BOD was determined
Remarks on result:
other: BOD
Parameter:
BOD5
Value:
0 mg O2/g test mat.
Results with reference substance:
The BOD5 of Sodium benzoate, corrected by the blank control is:
for 4 mg/l reference substance = 1275 mg/g O2
for 5 mg/l reference substance = 1280 mg/g O2
Validity criteria fulfilled:
not specified
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
The BOD of FAT 20004/H is 0 mg O2/g test mat.
Executive summary:

The biochemical oxygen demand of FAT 20004/H is determined by measuring the dissolved oxygen concentration before and after 5 days incubation at 20 °C in different test concentrations diluted in water saturated with dissolved oxygen that contains a seed of micro-organisms. The test was carried out according to ISO 5815 Second Edition 1989-08-01 (E) corresponding to 92 / 69 / EEC C.5. The test substance was tested in concentrations from about 1 to 500 mg/I. The reference substance used was Sodium benzoate (>99,5 %, FLUKA Nr. 71295). The BOD of FAT 20004/H is 0 mg O2/g test mat.


The BOD5 of Sodium benzoate, corrected by the blank control is: for 4 mg/l reference substance = 1275 mg/g O2; for 5 mg/l reference substance = 1280 mg/g O2. In another study, the BOD for FAT 20004/H was determined to be 1488 mg/g O2. The resultant BOD:COD ratio was calculated to be zero, indicating FAT 20004/H is not biodegradable.

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Experiment start and end date - 08 November 1990; Study completion date - 04 February 1991.
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
other: EEC Directive 79-831, Annex V, C.9. according to German DIN-Norm 38*409, H-41, 1 (Dezember 1980)
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
Name: FAT 20004/H
Storage: room temperature
Appearance: solid
Oxygen conditions:
other: Titration method
Inoculum or test system:
other: Titration method
Duration of test (contact time):
2 h
Parameter followed for biodegradation estimation:
test mat. analysis
Reference substance:
other: KHP (Merck No.4876)
Remarks on result:
other: COD was determined
Parameter:
COD
Value:
1 488 mg O2/g test mat.
Results with reference substance:
The COD of reference substance is 196 mg O2/g test mat.
Validity criteria fulfilled:
not specified
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
The COD of FAT 20004/H is 1488 mg O2/g test mat.
Executive summary:

A key study was performed to determine the chemical oxygen demand for FAT 20004/H according to DIN 38409 - H 41-1 (1980) corresponding to EEC Directive 92 / 69 / C.6. (1992). The reference substance was KHP (Merck No.4876). It was tested with 2.5 mg of the test substance. The COD of FAT 20004/H is :1488 mg O2/g test mat. The COD of the reference substance is: 196 mg O2/g test mat. In another study, the BOD of FAT 20004/H was determined to be 0 mg O2/g. The resultant BOD:COD ratio was found to be 0 indicating that the substance is not biodegradable.

Description of key information

The test substance FAT 20004 can be regarded as non-biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

The inherent biodegradability of the test substance FAT 20004/H was determined in a GLP-compliant study in Zahn-Wellens / EMPA Test according to OECD Guideline 302B. The test substance FAT 20004/H and the reference substance Diethylene glycol, respectively, were tested. The biodegradation of the test substance FAT 20004/H after 28 days was 97.75 %. Individual BOD5 and COD estimation were done in GLP-compliant studies according to ISO 5815 and EEC 92/69/C.6 (1992), respectively. The biochemical oxygen demand of FAT 20004/H was determined by measuring the dissolved oxygen concentration before and after 5 days incubation at 20 °C in different test concentrations diluted in water saturated with dissolved oxygen that contains a seed of micro-organisms. The BOD5 and COD of FAT 20004/H was found to be 0 mg O2/g and 1488 mg O2/g, respectively. Few non-GLP compliant studies performed in the 70’s have reported the following BOD5 and COD values for FAT 20004:


FAT 20004/A: BOD5 = 14 mg/g O2; COD = 1182.40 mg/g O2


FAT 20004/B: BOD5 = 22.5 mg/g O2; COD = 1389.12 mg/g O2


FAT 20004/C: BOD5 = 53 mg/g O2; COD = 1215.16 mg/g O2


FAT 20004/D: BOD5 = 11.4 mg/g O2; COD = 1274.01 mg/g O2


BOD5 = 11 mg/g O2; COD = 933.54 mg/g O2


Relevant is the BOD5/COD quotient which is not >0.5, therefore the substance is not indicative of rapid degradation. Taking into consideration the data from key study and supporting studies, the test substance FAT 20004 can be regarded as non biodegradable.