Disodium 5,5'-[(1-methylethylidene)bis(4,1-phenyleneoxysulphonyl-2,1-phenyleneazo)]bis[6-amino-4-hydroxynaphthalene-2-sulphonate]

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Study completion date - 04 July 1975.

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

CIBA internal Method A-11

Deviations:

no

Principles of method if other than guideline:

Groups of eight fishes were exposed to the test substance for 48 h at 100, 200 and 1000 mg/L each.

GLP compliance:

no

Specific details on test material used for the study:

Name: FAT 21021/A
Purity: 64.01 %

Analytical monitoring:

no

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

Not available

Test temperature:

14 °C

pH:

7.9-8.3

Nominal and measured concentrations:

100, 200 and 1000 mg/L

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

400 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

At 100 mg/L: No mortality was observed.
At 200 mg/L: 7/8 fish survived.
At 1000 mg/L: 0/8 fish survived.

Validity criteria fulfilled:

no

Conclusions:

The LC50 of FAT 21021/A in fish acute toxicity test was determined to be 400 mg/L.

Executive summary:

An acute fish toxicity test was performed with Rainbow trout species according to CIBA internal method A-11. 8 fish each were exposed at the test concentrations of 100, 200 and 1000 mg/L for a period of 48 h. No mortality was observed at 100 mg/L, while 1/8 and 8/8 fish were found dead at 200 and 1000 mg/L, respectively. Based on the findings of this study, the LC50 of FAT 21021/A in fish acute toxicity test was determined to be 400 mg/L.

Description of key information

An acute fish toxicity test was performed with Rainbow trout species according to CIBA internal method A-11. Eight fishes each were exposed at the test concentrations of 100, 200 and 1000 mg/L for a period of 48 h. No mortality was observed at 100 mg/L, while 1/8 and 8/8 fish were found dead at 200 and 1000 mg/L, respectively. Based on the findings of this study, the LC50 of FAT 21021/A in fish acute toxicity test was determined to be 400 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

400 mg/L