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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Jul - Aug 1994
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: According to OECD guideline under GLP
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference Type:
study report
Report date:

Materials and methods

Test guideline
according to guideline
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Principles of method if other than guideline:
Not relevant
GLP compliance:

Test material

Constituent 1
Chemical structure
Reference substance name:
EC Number:
EC Name:
Cas Number:
Molecular formula:
Details on test material:
- Name of test material (as cited in study report): Ethyldienon
- Analytical purity: 89.1%
- Lot/batch No.: 21400404

Study design

Oxygen conditions:
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
10 mg/L
Based on:
test mat.
Initial conc.:
20 mg/L
Based on:
test mat.

Results and discussion

% Degradation
% degradation (CO2 evolution)
0 - 2
Sampling time:
28 d
Details on results:
The reference compound sodium acetate was degraded to 100%, the test compound
Ethyldienon was degraded to 2%. The variation in the degradation is within the accuracy of the method.

Any other information on results incl. tables

Table 1: Biological degradation (cumulative) in percent

   Day of sampling  
 Test compound    Nominal concentration 3 4 7 11 16 21 25 29
 acetate    20 mg/L  18 31 54 80 96 102 105 105
 ZK 28519    10 mg/L 1 1 1 2 0 0 1 0
 ZK 28519    20 mg/L  0 0 0 0 0 0 1 2

Applicant's summary and conclusion

Validity criteria fulfilled:
Interpretation of results:
inherently biodegradable
In accordance with the OECD guideline, the test product Ethydienon is not readily biodegradable under the conditions of the test.
Executive summary:

The purpose of this study was to determine the ready biodegradability of the product Ethyldienon (ZK 28519). The study was conducted in agreement with the following test guideline: OECD guidelinefor testing of chemieals: Ready biodegradability: Modified Sturm test, No. 301 B, adopted May 1981.

The test substance was incubated in aqueous solution including nutrients with microorganisms fram a municipal sewage treatment plant for 28 days (start of treatment = day 1). The nutrient solutions were made up of phosphates, ammonium sulphate, magnesium sulphate, iron chloride, ammonium chloride and calcium chloride, and added to the test solution. Additionally, a reference substance (sodium acetate) was tested according to the same procedure, in order to verify the viability and activity of the degrading microorganisms. The test substance was incubated in two concentrations (10 mg/L and 20 mg/L suspension) and the reference substance in a concentration of 20 mg/L. Furthermore, one additional vessel without any test or reference substance was used as blank (control). The biological degradation of the test and reference substances was evaluated by measurement of the CO2 produced during the test period. CO2-production was determined on days 3, 4, 7, 11, 16, 21, 25, and 29 and calculated as the percentage of total CO2 that the test material could theoretically have produced, based on carbon content. The blank CO2 - production was subtracted for correction.

The test compound ZK Ethyldienon was degraded to 2%.