Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Jun 1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: According to OECD guideline under GLP
Cross-referenceopen allclose all
Reason / purpose:
reference to same study
Reason / purpose:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report Date:
1994

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Principles of method if other than guideline:
not relevant
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Ethyldienon
- Analytical purity: 89.1%
- Lot/batch No.: 21400404

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 2 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Saturated Solution

Any other information on results incl. tables

No immobilized Daphnia was observed in the control and test solutions.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
A saturated solution (2 mg/L) of ZK 28519 did not produce immobilization in Daphnia magna .
The EC50/48 hours of ZK 28519 to Daphnia magna was higher than 100 mg/L (nominal concentration).
Executive summary:

The purpose of this study was to determine the acute toxicity (immobilization, EC50/48 hours) of the product Ethyldienon (ZK 28519) with Daphnia magna in a limit test in order to evaluate the aquatic toxicity. The study was conducted in agreement with the guideline of the OECD, no. 202.

Twenty Daphnia were used for the control group and fourty Daphnia for the test solutions. The Daphnia were exposed to the test solutions and the dilution water control for aperiod of 48 hours under static conditions. Immobilization was recorded at 24 hours and 48 hours. The concentration of the filtered solution was analysed with a TOC-analyzer at 0 and 48 hours. The concentration was below the limit of detection (1-2 mg/L organic carbon).

No immobilized Daphnia was observed in the control and test solutions.