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Description of key information

Key value for chemical safety assessment

Acute toxicity: via oral route

Link to relevant study records
Reference
Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
July 2015
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP, Guideline study
Qualifier:
according to
Guideline:
OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Test type:
acute toxic class method
Species:
rat
Strain:
Wistar
Sex:
female
Route of administration:
oral: gavage
Vehicle:
CMC (carboxymethyl cellulose)
Remarks:
1% solution
Doses:
A limit dose of 2000 mg/kg was used as starting dose. The required amount of the test item (according body weight) was mixed with vehicle (carboxymethyl cellulose - 1% solution) shortly before administration.
No. of animals per sex per dose:
Groups of 6 females were dosed (Group of 3 females were dosed. Test item-related mortality was not produced during 24 hours group of 3 females were tested at the same dose).
Sex:
female
Dose descriptor:
LD0
Effect level:
>= 5 000 mg/kg bw

All 6/6 females survived the limit dose 2000 mg/kg.

No mortality was observed during the study. Animals lived through observation period without important visible signs of intoxication. Neither change of health nor negative reactions were registered.

The body weights of all animals were increasing during the study. No body weight losses were observed between one and two week after administration.

All animals were necropsied. During necropsy, no macroscopically changes were noticed.

Interpretation of results:
study cannot be used for classification
Remarks:
Migrated information
Conclusions:
Based on OECD 423 Annex 2d: Test Procedure with a Starting Dose of 2000 mg/kg Body Weight it can be concluded that the test item Zinc Hexacyanocobaltae Dodecahydrate is classified in Category 5/Unclassified with the cut off LD50 ≥ 5000 mg/kg, after single oral administration to Wistar rats.
Endpoint conclusion
Endpoint conclusion:
no adverse effect observed
Dose descriptor:
LD50
Value:
5 000 mg/kg bw

Acute toxicity: via inhalation route

Endpoint conclusion
Endpoint conclusion:
no study available

Acute toxicity: via dermal route

Endpoint conclusion
Endpoint conclusion:
no study available

Additional information

Justification for selection of acute toxicity – oral endpoint
One study available.

Justification for classification or non-classification

Based on OECD 423 Annex 2d: Test Procedure with a Starting Dose of 2000 mg/kg Body Weight it can be concluded that the test item Zinc Hexacyanocobaltae Dodecahydrate is classified in Category 5/Unclassified with the cut off LD50 ≥ 5000 mg/kg, after single oral administration to Wistar rats.