Guerbet alcohols, C24-26, branched and cyclic

Toxicity to terrestrial plants

Link to relevant study record(s)

Reference

Endpoint:

toxicity to terrestrial plants: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July to August 2020

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 208 (Terrestrial Plants, Growth Test)

Version / remarks:

adopted July 19, 2006

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

no

Details on preparation and application of test substrate:

- Method of mixing into soil (if used): The test concentration of 1000 mg/kg dw was prepared for two pieces of 4750 g. 4.7503 and 4.7501 g test item were weighed and mixed with 50 g soil, respectively.These mixtures was mixed with 4700 g dry soild each to be homogenous with the blender to get 1000 mg/kg dw. For the main test with pea 5500 g soil mixtures of the concentration of 100, 211, 447, 946 and 2000 mg/kg/kg were prepared of 0.5503 g, 1.1607g, 2.4610 g5.2031 g and 11.0078 g test item with 50g and afterwards 5450 g dry soil.
- Controls: The blank control group was maintained under identical conditions in parallel, only without test application.

Species:

Triticum aestivum

Plant group:

other: Poaceae (Gramineae)

Details on test organisms:

Origin: Shenyang Agricultural University Seed Industry

Species:

Cucumis sativus

Plant group:

other: Cucurbitaceae

Details on test organisms:

Origin: Heiongjiang Harbin Seed Breeding Center

Species:

Pisum sativum

Plant group:

other: Fabaceae (Leguminosae)

Details on test organisms:

Origin: Shenyang Gaotian Seed Industry Co. Ltd.

Test type:

seedling emergence and seedling growth test

Study type:

laboratory study

Substrate type:

natural soil

Limit test:

yes

Total exposure duration:

16 d

Remarks:

For pea no limit test was performed.

Test temperature:

24-27 °C

pH:

6.57

Moisture:

Humidity: 68-87%

Details on test conditions:

TEST SYSTEM
- Test container (type, material, size): plastic pot with diameter of 15 cm and depth of 10 cm
- Amount of soil: 600 g (dry weight)
- No. of seeds per container: 5 (wheat), 2 (cucumber), 3 (pea)
- No. of replicates per treatment group: 4 (wheat), 4 (pea, 2 containers per replicate), 5 (cucumber, 2 containers per replicate)
- No. of replicates per control: 4 (wheat and pea), 5 (cucumber)

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Farmland Gongzhuling County of Changchun City, Jilin Province, not cultivated
- Pesticide use history at the collection site: no history of pesticide and fertlizer application for 1 year
- Soil texture (if natural soil)
- % sand: 30.0 %
- % silt: 44.8 %
- % clay: 25.2 %
- Organic carbon (%): 1.72 %
- Soil moisture content at start of the test: 1.42 %
- Storage (condition, duration): 2-8 °C


GROWTH CONDITIONS
- Photoperiod: 16 hours light, 8 hours darkness
- Light intensity and quality: 18100 Lux- 20100 Lux, cool white flourescen
- Relative humidity (%): 68-87 %
- Water source/type: tap water
- Interval of applications: daily
- Method of application: Water was absorbed from the bottom (tap water was added to the tray) after the seeds have been planted

RANGE FINDING STUDY
- Test concentrations: 10,100,1000 mg/kg dw
- Results used to determine the conditions for the definitive study:
- Wheat: 1.7 %, 4.9 %, 4.1 % inhibition
- Cucumber: 8.1 %, 8.7, 6.8 % inhibition
- Pea: 6.3 %, 12.3 %, 46.3 % inhibition

Nominal and measured concentrations:

Wheat and cucumber: 1000 mg/kg dw
Pea: 100, 211, 447, 946 and 2000 mg/kg dw

Reference substance (positive control):

no

Key result

Species:

Triticum aestivum

Duration:

16 d

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

germination

Key result

Species:

Triticum aestivum

Duration:

16 d

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Key result

Species:

Cucumis sativus

Duration:

16 d

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

germination

Key result

Species:

Cucumis sativus

Duration:

16 d

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Key result

Species:

Pisum sativum

Duration:

16 d

Dose descriptor:

NOEC

Effect conc.:

2 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

germination

Key result

Species:

Pisum sativum

Duration:

16 d

Dose descriptor:

EC50

Effect conc.:

2 089 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks on result:

other: EC50 = 1030-4238 mg/kg dw

Details on results:

Measured concentrations at beginning of the test were 86.1 - 94.6% of nominal concentrations.
No visible phototoxic effects (e. g. chlorosis, necrosis, wilting, leaf and stem deformations). Plants exhibited only normal variation in growth and morphology for each particular species.

Reported statistics and error estimates:

The result of the Two-sided Student t-test indicated that the emergence of three species did not display significant differences (p> 0.05) between the control and each test group.
For wheat and cucumber, the dry shoot weight did not display significant differences (p> 0.05) between the control and the test group of 1000 mg/kg dw.
For pea, the dry shoot weight showed a visible concentration-dependent decrease.

Table 1: Germination, survival plants and plant dry weight for Wheat

Concentration	Mean germination rate	Inhibition rate of germination	p-value (t-test)	Mean survival rate	inhibition rate of survival	Average dry weight per shoot (g)	Inhibition rate to weight (%)
Control	95	-	-	100	-	0.0854	-
1000 mg/kg dw	100	- 5.3	0.36	100	0.0	0.0963	-11.6

Table 2: Germination, survival plants and plant dry weight for Cucumber

Concentration	Mean germination rate	Inhibition rate of germination	p-value (t-test)	Mean survival rate	inhibition rate of survival	Average dry weight per shoot (g)	Inhibition rate to weight (%)
Control	95	-	-	100	-	0.2810	-
1000 mg/kg dw	100	5.0	0.35	100	0.0	0.2578	8.3

Table 3: Germination, survival plants and plant dry weight for Pea

Concentration	Mean germination rate	Inhibition rate of germination	p-value (t-test)	Mean survival rate	inhibition rate of survival	Average dry weight per shoot (g)	Inhibition rate to weight (%)
Control	87	-	-	100	-	0.1599	-
100 mg/kg dw	83	4.6	0.62	100	0.0	0.1711	-7.0
211 mg/kg dw	79	9.2	0.21	100	0.0	0.1565	2.1
447 mg/kg dw	75	13.8	0.10	100	0.0	0.1366	14.6
946 mg/kg dw	79	9.2	0.21	100	0.0	0.1169	26.9
2000 mg/kg dw	75	13.8	0.1	100	0.0	0.0914	42.8

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of this test the test substance did not show terrestrial toxicity on the three species Wheat (Triticum aestivum), Cucumber (Cucumis sativus) and Pea (Pisum sativum).

Executive summary:

A terrestrial plant test with Guerbet alcohols, C24 -26, branched and cyclic was conducted with three plant species (wheat, cucumber and pea) according to OECD Guideline 208. For wheat and cucumber a limit test was performed with 1000 mg/kg dw. For pea the test was carried out with the following test concentrations: 100, 211, 447, 946 and 1000 mg/kg dw.

For the three plant species the seedlings didn't exhibit visible phytotoxic effetcs and the plants exhibited only normal variation in growth and morphology for that particular species.

For wheat and cucumber the dry shoot weight did not display significant differences (p> 0.05) between the control and the test groups of 1000 mg/kg dw. For pea a dose-related decrease of dry shoot weight was observed. According to the test results the effective concentrations of the test substance to the three terrestrial plants was: NOEC (germination) > 1000 mg/kg dw and EC50 (dry shoot weight) > 1000 mg/kg dw.

Description of key information

A terrestrial plant test with Guerbet alcohols, C24 -26, branched and cyclic was conducted with three plant species (wheat, cucumber and pea) according to OECD Guideline 208. For Wheat and cucumber a limit test was performed with 1000 mg/kg dw. For pea the test was carried out with the following test concentrations: 100, 211, 447, 946 and 1000 mg/kg dw.

For the three plant species the seedlings didn't exhibit visible phytotoxic effetcs and the plants exhibited normal variation in growth and morphology for that particular species.

For wheat and cucumber the dry shoot weight did not display siginifcant differences (p> 0.05) between the control and the test groups of 1000 mg/kg dw. For pea a dose-related decrease of dry shoot weight was observed. According to the test results the effective concentrations of the test substance to the three terrestrial plants was: NOEC (germination) > 1000 mg/kg dw and EC50 (dry shoot weight) > 1000 mg/kg dw.

Key value for chemical safety assessment

Short-term EC50 or LC50 for terrestrial plants:

1 000 mg/kg soil dw

Long-term EC10, LC10 or NOEC for terrestrial plants:

1 000 mg/kg soil dw