Guerbet alcohols, C24-26, branched and cyclic

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

September 2019 - January 2020

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Version / remarks:

April 4, 1984

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

no

Details on preparation and application of test substrate:

- Method of mixing into soil: The test item was mixed with industrial sand. The treated sand was mixed thoroughly with proper artificial soil. After mixing distilled water was added to adjust the moisture content to about 30 % of dry weight and the sample was mixed to be homogeneous.
- Controls: Maintanance under identical consitions without test item application.

Test organisms (species):

Eisenia fetida

Details on test organisms:

TEST ORGANISM
- Common name: Earthworm
- Source: Chenggong Earthworm Raising Farm, Dongli District, Tianjin
- Age at test initiation (mean and range, SD):at least two months old with clitellum
- Weight at test initiation (mean and range, SD): 0.3350 - 0.5344 g

ACCLIMATION
- Acclimation period: 24 h
- Acclimation conditions (same as test or not):same as test

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

yes

Total exposure duration:

14 d

Test temperature:

18.2 - 21.2 °C

pH:

6.22 - 6.28

Details on test conditions:

TEST SYSTEM
- Amount of soil: 750 g
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- % sand: 70
- % sphagnum peat: 10
- % clay: 20

OTHER TEST CONDITIONS
- Photoperiod: continuous light
- Light intensity: 538-631 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality (days 7 and 14), body weight, soil moisture content and soil pH (start and end of treatment)

TEST CONCENTRATIONS
- Range finding study: 1000 mg/kg dw
- Test concentrations: 1000 mg/kg dw

Nominal and measured concentrations:

Nominal: 0, 1000 mg/kg dry soil

Reference substance (positive control):

yes

Remarks:

cloracetamide, historical control

Key result

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Key result

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

>= 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- Mortality at end of exposure period: no mortality
- Changes in body weight of live adults (% of initial weight) at end of exposure period: 92.2 (controls), 92.6 (tretament group)

Validity criteria fulfilled:

yes

Conclusions:

Accoring to the result, the 14d LC50 of the test item to earthworms (Eisenia foetida) was greater than nominal 1000 mg/kg dw under the test conditions.

Executive summary:

To investigate the toxicity of the test substance to soil macroorganisms a study on earthworms was performed according to OECD Test Guideline 207. 4 replicates of 10 earthworms each were exposed in artificial soil to 1000 mg/kg dw of the test substance for 14 d ays. No mortality or abnormal signs were observed. According to the result, the 14d LC50 of the test item to earthworms (Eisenia foetida) was greater than nominal 1000 mg/kg dw under the test conditions.

Description of key information

The toxicity of the test substance to soil macroorganisms was investigated in a study on earthworms performed according to OECD Test Guideline 207. 4 replicates of 10 earthworms each were exposed in artificial soil to 1000 mg/kg dw of the test substance for 14 d ays. No mortality or abnormal signs were observed. According to the results, the LC50 (14 d) of the test item to earthworms (Eisenia foetida) was greater than nominal 1000 mg/kg dw under the test conditions.

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for soil macroorganisms:

1 000 mg/kg soil dw