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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- - Name of test material (as cited in study report): Gelb LD 259
- Lot/batch No.: 0817VP01
- Analytical purity: 87.6 - 89.1 area %
- Physical state: solid
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Each test solution was prepared seperately. The test substance was added to 5 l test medium which was subsequently stirred for 5 minutes at 20 +/- 2 °C.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: CHODAT SAG 86.81
- Source (laboratory, culture collection): Collection of algal cultures in Goettingen, Germany)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 24 °C
- pH:
- 8.1 - 8.6 (control
7.8 - 8.5 (treatments) - Nominal and measured concentrations:
- Nominal test concentrations (mg/l):
control, 10, 22, 46, 100 and 220
The analytically determined concentrations were within 20 % of the nominal concentration.
Analytically determined concentrations (mg/l):
10.1, 22.2, 46.5, 101.6, 225.4 - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer vessels (250ml) set up in a controlled climate cabinet (Vötsch Industrietechnik GmbH Bioline (VB1014))
- Type (delete if not applicable): test vessels were plugged with a gaspermeable silicon sponge cap
- Fill volume: 50 ml
- Initial cells density: 300 cells/ml
- Control end cells density: 870000 cells/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to OECD guideline No. 201
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: permanent illumination
- Light intensity and quality: artificial light, (OSRAM L25), average 6892 lux, wave length 400-700 nm
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: microscopic cell counting in a Neubauer haemocytometer
Mean fluorescence was determined after 0, 24, 48 and 72 hours
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2
- Results used to determine the conditions for the definitive study: EC50 (growth rate) > 100 mg/l - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 22 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 220 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- The ErC50(72h) of the reference substance potassium dichromate was 0.86 mg/l.
(Date of the last control experiment: 20 Jan 2009, project-no. 60E0063/043030)
Any other information on results incl. tables
Nominal concentration [mg/l] |
Mean cell density (cells/ml) x 104at 72 h |
% inhibition (as yield) |
% inhibition (as growth) |
0 |
87 |
0.0 |
0.0 |
10 |
83 |
4.63 |
1.04 |
22 |
82 |
5.79* |
1.33 |
46 |
56 |
36.7** |
10.1** |
100 |
40 |
54.4** |
17.3** |
220 |
32 |
64.1** |
22.5** |
*p<=0.05; **p<=0.01
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In conclusion the 72h ErC50 for Gelb LD 6259 with Desmodesmus subspicatus were >220 mg/L based on the nominal concentration of the test substance and >224.4 mg/L based on the mean of analytically determined concentrations.
- Executive summary:
The test substance inhibited algal growth in a concentration dependent manner. However this effect is likely due to the strong color of the test solutions imparted by the test substance. The OECD 23 guidance document recogbnizes that coloured substances can absorb light and thus limit photosynthetic growth of algal cultures proportionally to test substance concentration. Consequently, this physical effect on algal growth is difficult to distinguish from inherent toxicity. The experimental system in this study was modified slightly in order to compensate for the physical effect of color, however this confounding effect can not be completely eliminated. Hence, the results of this study should be interpreted judiciously.
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