Registration Dossier
Registration Dossier
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EC number: - | CAS number: -
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- - Name of test material (as cited in study report): Gelb LD 6259
- Lot/batch No.: 0817VP01
- Analytical purity: The IR spectrum shows the expected bands; the 1H and 13C NMR spectra show expected signals for the current structure (BASF report No. 08L00346
- Physical state: solid
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): municipal activated sludge taken from the waste water treatment plant of Mannheim (Baden-Wuertemberg, Germany).
- Preparation of inoculum for exposure: the inoculum was sieven (mesh size 1 mm) and pre-aerated
- Concentration of sludge: 30 mg/l
Initial test substance concentrationopen allclose all
- Initial conc.:
- 86 mg/L
- Based on:
- test mat.
- Initial conc.:
- 20 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Test temperature: 22 +/- 2 °C
TEST SYSTEM
- Number of culture flasks/concentration: 2
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- < 10
- Sampling time:
- 28 d
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: poorly biodegradable
- Conclusions:
- The test substance was poorly biodegradable and according to OECD criteria "not readily biodegradable " in this test.
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