Registration Dossier
Registration Dossier
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EC number: 215-181-3 | CAS number: 1310-58-3
Toxicity to soil macroorganisms except arthropods
Administrative data
- Endpoint:
- toxicity to soil macroorganisms except arthropods: long-term
- Adequacy of study:
- supporting study
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: no guideline followed
Data source
Reference
- Reference Type:
- publication
- Title:
- The influence of some acids, bases and salts on an enchytraeid population of a pine litter substrate
- Author:
- Heungens A
- Year:
- 1 984
- Bibliographic source:
- Pedobiologia, 26, 137-141
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Test substance was added to fresh pine litter substrate. pH and conductivity was measured during 6 months. At regular times the enchytraeids are counted both in the controls and in the test samples. Toxicity was estimated by calcuting the regression equation between reproduction number and pH or conductivity.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Potassium hydroxide
- EC Number:
- 215-181-3
- EC Name:
- Potassium hydroxide
- Cas Number:
- 1310-58-3
- Molecular formula:
- HKO
- IUPAC Name:
- potassium hydroxide
- Details on test material:
- - Name of test material (as cited in study report): KOH
- Physical state: liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
- Details on sampling:
- - Concentrations: 0, 500, 1000, 1500, 2000, 2500, 3000, 3500, 4000, 4500, 5000 mg/l
Test organisms
- Test organisms (species):
- Enchytraeus sp.
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Source: Rijksuniversiteit Gent
Study design
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Total exposure duration:
- 180 d
Test conditions
- pH:
- 7.3 (init.) - 6.3 (fin.)
- Moisture:
- humidity was adjusted weekly with rainwater
- Details on test conditions:
- TEST SYSTEM
- Amount of soil or substrate: 3 g/l
- No. of replicates per treatment group:4
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
-Composition (if artificial substrate): fresh pine substrate
- pH=4.4
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : reproduction - Nominal and measured concentrations:
- - Nominal concentrations: 0, 500, 1000, 1500, 2000, 2500, 3000, 3500, 4000, 4500, 5000 mg/l
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 180 d
- Dose descriptor:
- EC50
- Effect conc.:
- 850 other: mg/L
- Nominal / measured:
- estimated
- Basis for effect:
- other: population density
- Duration:
- 180 d
- Dose descriptor:
- other: EC92
- Effect conc.:
- 3 000 other: mg/L
- Nominal / measured:
- estimated
- Basis for effect:
- other: population density
Any other information on results incl. tables
The values of LC50 and LC92 are calculated using the regression given in the method. The enchytraeid population was >95% Cognetia sphagnetorum.
Applicant's summary and conclusion
- Conclusions:
- 90d LC50 = 850 and LC92 = 3000 mg/l for the enchytraeid population in fresh pine substrate.
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