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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2003
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Remarks:
Guideline study without GLP. The test report is lacking in experimental detail but the test was conducted in accordance with a recognised guideline.
Qualifier:
according to guideline
Guideline:
ISO 8692 (Water Quality - Fresh Water Algal Growth Inhibition Test with Scenedesmus subspicatus and Selenastrum capricornutum)
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION

- Method: Test media were prepared by dilution of a stock solution that had been prepared by 28.5x dilution of the substance.
Test organisms (species):
Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Details on test organisms:
No data
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Hardness:
No data
Test temperature:
No data
pH:
8.2 at start of test
Dissolved oxygen:
No data
Salinity:
No data
Nominal and measured concentrations:
No data
Details on test conditions:
- Adjustment of pH: the pH was adjusted to 8.2 at the start of the test
Reference substance (positive control):
no
Key result
Duration:
72 h
Dose descriptor:
EL50
Effect conc.:
0.03 other: vol. %
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: growth inhibition
Key result
Duration:
72 h
Dose descriptor:
EL50
Effect conc.:
309 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
assuming density of sample is 1030 mg/ml
Basis for effect:
other: growth inhibition
Details on results:
No data
Reported statistics and error estimates:
No data
Validity criteria fulfilled:
yes
Conclusions:
A 72-hour EL50 of 310 mg/L (0.03 vol. %) has been determined for the effects of the test substance on growth of Pseudokirchnerella subcapitata.

Description of key information

A 72-hour EL50 of 309 mg/L has been determined for the effects of the registered substance on growth of Pseudokirchneriella subcapitata. A second test gave the same results. Test results are also available for a closely related substance, Crude Tall Oil (EC number 931-433-1). A 72-hour EL50 of > 100 mg/L and NOELR of ≥100 mg/L were determined in the test for effects on growth of Pseudokirchnerella subcapitata. It is therefore concluded that the 72-hour EL50 for the substance is >100 mg/L and probably 309 mg/L.

Key value for chemical safety assessment

Additional information

TOS is a UVCB substance and compositional information has been used to derive No Observed Effect Concentrations (NOECs) for its constituent blocks. Calculation of long-term PNECs for the aquatic compartment will be based on data for the constituent blocks rather than on data for the whole substance. Further testing for long-term toxicity to aquatic algae will therefore not contribute to the database that is required to complete the assessment of the substance.