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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Ethylbenzene was evaluated for biodegradation, as per procedures laid out in the ISO 14593 - CO2 Headspace Test. The test conditions were equivalent to the OECD 301 B: CO2 Evolution (Modified Sturm Test). Ethylbenzeneshowed 79% degradation in this study within the 10 day window and is therefore considered "readily biodegradable".

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

Ethylbenzene was evaluated for biodegradation, as per procedures laid out in the ISO 14593 - CO2 Headspace Test. The test conditions were equivalent to the OECD 310: (CO2 in sealed vessels [Headspace Test])(BASF AG, 2003). The kinetics of ethylbenzene at the end of 28 days revealed levels of up to 79% biodegradation based on the mineralization of the test material.

This level of biodegradation was reached within the prescribed 10-day window. The 10-day window for ethylbenzene started on day 2 and ended on day 12 where biodegradation reached approximately 66% which exceeded 60% criteria for CO2production. Thus, ethylbenzene was classified as readily biodegradable.

Additional supporting studies demonstrated that ethylbenzene was also inherently biodegradable based upon the OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II)(Chemicals Inspection and Testing Institute, 1972). Finally there were numerous supporting studies available in the scientific peer reviewed literature demonstrating that ethylbenzene degraded aerobically and anaerobically in natural groundwater environments and in domestic wastewater treatment plants.