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Ecotoxicological information

Long-term toxicity to fish

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Description of key information

28-d NOECs concerning mortality are available for acenaphthene as a key component and marker substance of wash oil (composite sample No. 05) for freshwater and marine fish; they are of the same order of magnitude with the freshwater NOEC somewhat lower.

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater fish:
320 µg/L
EC10, LC10 or NOEC for marine water fish:
520 µg/L

Additional information

No experimental data is available for creosote oil, acenaphthene fraction (wash oil) itself. Thus, information from two constituents of wash oil, the marker substance acenaphthene (see Chapter 7.1.) and the substance fluorene, is used as substitute in order to characterise fish long-term toxicity of wash oil. Tests were performed in freshwater (acenaphthene and fluorene) and in saltwater (acenaphthene).

Freshwater data (measured concentrations) were determined for Pimephales promelas (fathead minnow) and Lepomis macrochirus (blue gill) in flow-through systems with acenaphthene and fluorene as test substances (see table above). Acenaphthene NOEC of two tests (32 d and 34 d) ranged from 332 µg/L to 509 µg/L (larval development) with a 28 d EC10/NOEC of >320 - <560 µg/L (neonate mortality) (Cairns and Nebeker, 1982). The 30-d NOEC for fluorene was 42 µg/L (growth rate) (Finger et al., 1985).

A 28-d NOEC of 520 µg/L was presented by Ward et al. (1981) for the marine fish Cyprinodon variegatus (sheepshead minnow) in a flow-through test. The value (measured concentration) is based on juvenile fish mortality.

Long-term fish toxicity of fluorene was considerably higher than that of acenaphthene (lower NOEC, see above).