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EC number: 619-079-3 | CAS number: 949109-75-5
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
Biodegradation in water: screening tests: 90-92% in 28 days (BODIS). Readily biodegradable.
Key value for chemical safety assessment
Additional information
90-92% biodegradation was attained in 28 days in a reliable BODIS study. The 10-day window is not relevant for substances such as this that are a mixture of homologous compounds (ECHA, 2008). The substance is readily biodegradable.
A BODIS test (Biological Oxygen Demand of Insoluble Chemicals), or two-phase closed bottle test, is a method developed to investigate the ultimate aerobic degradability of poorly soluble chemicals (SCHER, 2005). BOD bottles and an oxygen electrode are used as in the OECD 301D Closed Bottle Test. One third of the bottle (headspace) acts as an oxygen reservoir. Consequently, higher test substance concentrations than in the OECD 301D test can be used, as the oxygen concentration in the (saturated) medium is no longer the limiting factor for degradation. Concentrations of up to 100 mg COD/l can be weighed directly into the test flasks.
A comparison of the biodegradation results obtained with the ISO 10708 to those acquired by other ready biodegradation methods has been carried out (SCHER, 2005). It was concluded that using the biodegradability pass criterion of the 60% BOD/COD the biodegradation results obtained with the BODIS test are consistent (i.e. results in a similar final evaluation) with those obtained with the OECD ready biodegradability methods.
The phytosterol used in the study was refined phytosterol extracted from soybean oil. The concentration of test substance was 100 mg COD/l.
In two independent series of tests reliably high biochemical oxygen consumption rates were determined of 90 and 92% BOD/COD (the theoretical COD determined for the substance was2.94mgO2/mg test substance).
SCHER (2005) EUROPEAN COMMISSION. SCIENTIFIC COMMITTEE ON HEALTH AND ENVIRONMENTAL RISKS (SCHER) Opinion on “Compatibility of the ISO standard 10708 (biodegradability test method) with the ultimate biodegradability requirements imposed through Annex III of Regulation 648/2004 of Parliament and of the Council” Adopted by the SCHER during the 3rd plenary of 28 January 2005.
For completeness, a range of supporting studies from the open literature has been reviewed (see Appendix I of the CSR). It can be concluded from these studies that plant sterols degrade rapidly, although their fate in WWTP should (in the normal way for exposure assessment) take operational conditions into account.
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