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Toxicological information

Toxicity to reproduction: other studies

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Administrative data

Endpoint:
toxicity to reproduction: other studies
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Remarks:
The study provides scientific evidence that heparin binding autocrine factors modulate testosterone production by the adult rat Leydig cell.

Data source

Reference
Reference Type:
publication
Title:
Evidence that heparin binding autocrine factors modulate testosterone production by the adult rat Leydig cell
Author:
McFarlane JR, Laslett A, de Kretser DM and Risbridger GP
Year:
1996
Bibliographic source:
Molecular and Cellular Endocrinology 118: 57-63

Materials and methods

Principles of method if other than guideline:
Leydig cells obtained from testes of adult rats aged between 90-120 days, and purified (purity of the resulting Leydig cell preparations was 96 ± 3%). These cells were incubated in the absence or presence of a maximally stimulating dose of LH, together with the test substances (sodium chlorate, protamine sulphate, and sodium chlorate/heparin). Controls of nonspecific binding were included in the test.
GLP compliance:
not specified
Type of method:
in vitro

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Sodium Chlorate

Results and discussion

Effect levels

Remarks on result:
not measured/tested

Observed effects

The sodium chlorate (25 mM) inhibited testosterone production by LH stimulated cells by over 50%, but had no effect on unstimulated cells.
Testosterone production by dibutryl-cAMP stimulated Leydig cells was also inhibited by sodium chlorate.

Any other information on results incl. tables

The sodium chlorate (25 mM) inhibited testosterone production by LH stimulated cells by over 50%, but had no effect on unstimulated cells. Testosterone production by dibutryl-cAMP stimulated Leydig cells was also inhibited by sodium chlorate.

The LH responsiveness and testosterone production returned to normal after the sodium chlorate was removed from the culture media

Applicant's summary and conclusion

Conclusions:
The sodium chlorate (25 mM) was able to significantly suppress testosterone production by adult rats LH stimulated cells (over 50%) and also the testosterone production by dibutryl-cAMP stimulated Leydig cells.
Executive summary:

The aim of this study was to determine the role of cell surface heparan sulfate proteoglycans in the regulation of testosterone secretion by adult rat Leydig cells. In some experiments the effect of sodium chlorate was evaluated. The sodium chlorate (25 mM) inhibited testosterone production by LH stimulated cells by over 50%, but had no effect on unstimulated cells. Testosterone production by dibutryl-cAMP stimulated Leydig cells was also inhibited by sodium chlorate. The LH responsiveness and testosterone production returned to normal after the sodium chlorate was removed from the culture media.