Registration Dossier
Registration Dossier
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EC number: 700-371-5 | CAS number: 178535-25-6
Endpoint summary
Administrative data
Description of key information
Additional information
1 - 10 tonnes of Ethylbenzene, manuf. of, distn. residues, distn. lights. is imported into Europe per annum. The data requirements for this tonnage band require that a Ready Biodegradability test is carried out on the substance.
Preliminary work showed that the test material was volatile and hence the conditions employed for a Modified CO2 Evolution Test (OECD Guideline No. 301B) would not be suitable for the determination of the biodegradability of the test material. The test method was therefore changed to a CO2 Headspace test, which is recommended for volatile materials. This test was conducted in sealed vessels and assessed the biodegradation of a test material by measuring the inorganic carbon present in the headspace of the vessels.
The test material, at a concentration of 20 mg C/l, was exposed to activated sewage sludge micro-organisms with mineral salts medium in sealed culture vessels in the dark at 20±1.0°C for 28 days.
The degeneration of the test material was assessed by the determination of carbon dioxide produced on Days 0, 2, 4, 8, 10, 14, 16, 21 and 28. Control solutions with inoculum and the standard material, sodium benzoate, together with a toxicity control were used for validation purposes.
The mean TIC in the control vessels on Day 28 was 0.10 mg/l; equivalent to 5% of the organic carbon added initially as test material to the test vessels and therefore satisfied the validation criterion given in the Test Guideline. The test material attained 15% degradation after 28 days and hence cannot be considered to be readily biodegradable.
The toxicity control attained 41% degradation after 8 days and 44% degradation after 14 days thereby confirming that the test material was not toxic to the sewage treatment micro-organisms used in the study. Sodium benzoate attained 81% degradation after 14 days and 87% degradation 28 days thereby confirming the suitability of the inoculum and test conditions. All validity criteria was therefore met.
Ethylbenzene, manuf. of, distn. residues, distn. lights. did biodegrade within the 28 day test. Although not readily biodegradable Ethylbenzene, manuf. of, distn. residues, distn. lights. was not toxic to the micro-organisms and is expected to biodegrade over time.
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