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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Description of key information

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
0.013 mg/L

Additional information

The lowest chronic test result obtained for Daphnia magna was in a GLP-test according to the OECD TG 202. A NOEC of 0.0126 mg/l and a LOEC of 0.04 mg/l were determined for the endpoint reproduction in a GLP-test according to the OECD TG 202 (CERI, 1996). The results are based on nominal concentrations of test material, since analytical monitoring with HPLC was performed and the test concentrations were confirmed. The test was conducted under flow-through conditions.

Chronic toxicity towards Daphnia magna was investigated also in a test according to the Dutch Standardization Organization Method NEN 6502. A 21 d-EC50 of 2.2 mg/l was obtained for the endpoint immobilisation. Endpoints population growth and length were also observed. For the endpoint population growth, a LRCT value (lowest rejected concentration tested) of 0.1 mg/l was reported. This value is similar to a LOEC (lowest observed effect concentration). The test was conducted with 17 anilines. The test concentrations were represented by the means of one aniline (2,4-dichloroaniline): 0.01, 0.032, 0.1, 0.32, 1.0, 3.2, 10.0 mg/l. In the summary table only the LOECs of the test substances were given. For o-toluidine (LOEC 0.1 mg/l) a NOEC of 0.032 mg/l can be derived in respect to the tested concentrations.