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EC number: 202-429-0 | CAS number: 95-53-4
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
48 h-EC50 value on Daphnia magna is 0.52 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.52 mg/L
Additional information
Two reliable studies on acute toxicity of o-toluidine to the invertebrate Daphnia magna are available. Maas-Dieperveen reported a 48 hours EC50 value of 0.52 mg/L which is the most sensitive value. The test was performed according to the Dutch Standardization Organization Method NEN 6501 without analytical monitoring. However, ecotoxicity studies with analytical monitoring indicated that o-toluidine was stable in water, and constant exposure concentrations over the test duration were assumed.
Data from a further test revealed an EC50 value of 15.6 mg/L after 48 hours with the OECD 202 for Daphnia magna. This is a nominal concentration, as no analytical monitoring was mentioned in the article.
Additionally reliable tests to other aquatic invertebrates are presented. An 72 h-EC0 value of 100 mg/l is reported for Mytilus edulis and Crassostrea gigas. No analytical monitoring was performed.
o-Toluidine was assessed within the scope of the ICCA/HPV-program, and the test procedure described by Abe et al. (2001) and Bringmann and Kuehn (1959 -1982) were scored to be reliable for assessment. Following the state-of-the-art, these studies are now considered as invalid due to insufficient documentation and/or unsuitable test systems, e.g. an EC50 could not be determined from specific values and no reason is given; it is not clear how the EC0 was determined regarding extremely low test concentrations; concerning test conditions the amounts of vitamins in test media are unclear.
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