Benzenesulfonic acid, 4-C10-13-sec-alkyl derivs., compds. with ethanolamine

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Nov. 18, 2002-Dec. 19, 2002

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Marl-Ost municipal sewage treatment plant
- Initial cell/biomass concentration: 3.94 g/l dry matter

Duration of test (contact time):

29 d

Initial conc.:

34.3 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Test temperature: 19.7-21.9 degree C
- pH: 7.4-7.5
- Aeration of dilution water: yes, overnight
- Suspended solids concentration: 26.3 mg/L

TEST SYSTEM
- Culturing apparatus: 5000 ml Woullf flasks
- Number of culture flasks/concentration: 2 flasks

SAMPLING
- Sampling frequency: 0, 2, 5, 9, 12, 14, 21, 28, and 29 days

CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 flasks

STATISTICAL METHODS: Results were calculated using the EXCEL computer program.

Reference substance:

benzoic acid, sodium salt

Key result

Parameter:

% degradation (CO2 evolution)

Value:

85

Sampling time:

29 d

Details on results:

The test substance was 85% degraded on day 29.

Results with reference substance:

The reference substance was 89% degraded in 29 days.

% Degradation

Test Duration (days)	Sodium Benzoate	Test Vessel 1	Test Vessel 2	Test Vessel Mean
0	0	0	-1	-1
2	32	1	-5	-2
5	59	24	19	22
9	76	57	46	52
12	84	73	66	70
14	84	72	67	70
21	93	76	79	78
28	95	86	80	83
29	89	84	85	85

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The test substance was 85% degraded in 29 days, and is considered readily biodegradable.

Executive summary:

This study examined the biodegradability of the test substance. 34.3 mg/L of test substance was added to flasks containing activated sludge. The amount of CO2 evolved was then measured over the next 29 days. Sodium benzoate was used as a reference substance. The reference substance was 89% degraded in 29 days. Therefore the test is valid. The test substance was 85% degraded in 29 days. The test substance is readily biodegradable.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)

Principles of method if other than guideline:

ISO 7827, Directive 79/831, V

GLP compliance:

no

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):
activated sludge, domestic, non-adapted (community: Lambsheim, federal state: Baden Württemberg, Germany)

Duration of test (contact time):

21 d

Initial conc.:

20 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST SYSTEM
- Number of culture flasks/concentration: 2

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes

Reference substance:

aniline

Parameter:

% degradation (DOC removal)

Value:

> 90

Sampling time:

21 d

Interpretation of results:

readily biodegradable

Description of key information

MEA-LAS dissociates in water to its constituents MEA and LAS, both of which are readily biodegradable. MEA-LAS can therefore be considered to be readily biodegradable.

Study 1:

A study was conducted to examine the biodegradability of C10-13 LAS, sodium salt, according to OECD Guideline 301B, in compliance with GLP. 34.3 mg/L of test substance was added to flasks containing activated sludge. The amount of CO2 evolved was then measured over the next 29 d. Sodium benzoate was used as a reference substance. The reference substance was 89% degraded in 29 d, therefore the study was valid. The test substance was 85% degraded in 29 d and can be considered readily biodegradable (Anon, 2002).

Study 3:

A DOC die away test according to OECD Guideline 301A was conducted with MEA. The substance (20 mg/L in the test system) was degraded by >90% within 21 d and is therefore considered to be readily biodegradable (BASF, 1995).

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Type of water:

freshwater