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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Testing was conducted between 21 May 2009 and 25 June 2009
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Fully GLP and test guideline compliant study.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report date:
2009

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
3,3'-(hexane-1,6-diyldiimino)dipropanenitrile
EC Number:
700-129-9
Cas Number:
2004-62-8
Molecular formula:
C12H22N4
IUPAC Name:
3,3'-(hexane-1,6-diyldiimino)dipropanenitrile

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, adapted
Details on inoculum:
A mixed population of sewage sludge micro-organisms was obtained from the final effluent stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK, which treats predominantly domestic sludge.
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimationopen allclose all
Parameter followed for biodegradation estimation:
O2 consumption
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
The degredation of the test material was assessed by the measurement of daily oxygen consumption values and Dissolved Organic Carbon analysis on Days 0 to 28. Control solutions with inoculum and the standard material, aniline, together with a toxicity control were used for validation purposes.
Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Parameter:
% degradation (DOC removal)
Value:
3
Sampling time:
28 d
Details on results:
Samples for analysis were taken daily from the prepared test solutions.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
not inherently biodegradable
Executive summary:

The test material attained 3% degredation after 28 days and therefore cannot be considered as readily biodegradable under the strict terms and conditions of OECD Guideline No 301F.