3,3'-(hexane-1,6-diyldiimino)dipropanenitrile

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Testing was conducted between 01 June 2009 and 30 July 2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Fully GLP and test guideline compliant study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3,3'-(hexane-1,6-diyldiimino)dipropanenitrile

EC Number:

700-129-9

Cas Number:

2004-62-8

Molecular formula:

C12H22N4

IUPAC Name:

3,3'-(hexane-1,6-diyldiimino)dipropanenitrile

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples were taken for analysis at the start and end of the exposure period.

Test solutions

Vehicle:

yes

Details on test solutions:

100 and 32 mg of the test material were each separately dissolved in culture medium to give 100 and 32 mg/l stock solutions respectively. A series of dilutions was made from these stock solutions to give further stock dilutions of 10, 3.2 and 1.0 mg/l. An aliquot (900 ml) of each of the stock solutions was separately inoculated with 10.3 ml of aldal suspension to give the required test concentrations of 1.0, 3.2, 10, 32 and 100 mg/l.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

Obtained from the Culture Collection of Algae and Protozoa (CCAP), Dunstaffnage Marine Laboratory, Oban, Argyll, Scotland.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Hardness:

Not applicable.

Test temperature:

24 +/- 1 oC

pH:

Samples had a range of pH from 7.7 to 9.0 at the start and a range of 7.6 to 7.8 at the end of the study.

Dissolved oxygen:

Not applicable.

Salinity:

Not applicable.

Nominal and measured concentrations:

Nominal concentrations : 1.0, 3.2, 10, 32 and 100 mg/l

Measured test concentrations ranged from 81% to 114% of nominal.

Details on test conditions:

The study was conducted under constant illumination and shaking.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

76 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 4

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

19 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Yield

Remarks on result:

other: 95% CL 14 - 24

Effect concentrations 5

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Yield

Effect concentrations 6

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

3.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Yield

Details on results:

Measured environmental values were all in expected ranges.

Results with reference substance (positive control):

The results from the positive control with potassium dichromate were within the normal range for this reference material.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

The effect of the test material on the growth of Desmodesmus suspicatus has been investigated over a 72-Hour period gave the following results:

Response Variable	EC50 (mg/l)	95% Confidence Limits (mg/l)	No Observed Effect Concentration (NOEC) (mg/l)	Lowest Observed Effect Concentraction (LOEC) (mg/l)
Growth Rate	76	*	10	32
Yield	19	14 - 24	1.0	3.2

*It was not possible to calculate the 95% confidence limits for the ErC50 values as the data generated did not fit the models available for the calcluation fo the confidence limits.