3-chloropropane-1,2-diol

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

6 - 9 December 2004

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study has been performed according to the OECD guideline and according to GLP principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2005

Report date:

2005

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-chloropropane-1,2-diol

EC Number:

202-492-4

EC Name:

3-chloropropane-1,2-diol

Cas Number:

96-24-2

Molecular formula:

C3H7ClO2

IUPAC Name:

3-chloropropane-1,2-diol

Details on test material:

- Name of test material (as cited in study report): 3-chloro-1,2-propanediol
- Synonym: alphaMCG, glycerol-alpha-monochlorohydrine
- Substance type: light yellow to almost colourless viscous liquid
- Physical state: liquid
- Analytical purity: 99.3 % (GC)
- Purity test date: no data, re-test date February 2005
- Lot/batch No.: A013405101
- Storage condition of test material: room temperature
- Other: supplier: Acros Organics, 's-Hertogenbosch, The Netherlands

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: algal medium (0 mg/L), 100 mg/L nominal
- Sampling method: triplicate aliquots of 100 µL of stock solution and algal medium on day 0 and 1.0 mL aliquots of all test solutions on day 3
- Sample storage conditions before analysis: samples (from day 3) were centrifuged to remove algae. Aliquots of day 0 and 100 ul of the supernatant of day 3 samples were transferred into a HPLC vial, containing 900 ul of trichloropropane (5 mg/L) in methanol and stored at 2-8 deg C, maximum 5 days

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
* preparation of stock solution: 110 mg of 3-chloro-1,2-propanediol (alphaMCG) in 1 liter algal medium resulting in a concentration of 0.11 g/L
* test solution: 90 mL of stock solution and 10 mL of algal medium resulting in a concentration of 100 mg/L
- Controls: yes

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: freshwater green alga
- Strain: ATCC 22662
- Source (laboratory, culture collection): Culture collection of algae and protozoa, Ambleside, Cumbria, UK
- Method of cultivation: 100-mL Erlenmeyer flasks with 50 mL filter sterilised medium were used for algae culture. Each week 0.5-2 ml of the culture is transferred to fresh algal medium. The preculture used for the study was 4 days of age at the start of the test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Post exposure observation period:

not applicable

Test conditions

Hardness:

no data

Test temperature:

23.8-24.3 deg C

pH:

7.7-8.9

Dissolved oxygen:

no data

Salinity:

not applicable

Nominal and measured concentrations:

nominal concentrations: 0 mg/L and 100 mg/L
measured concentrations: below detection limit and 97.4-105 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 100-mL Erlenmeyer flasks randomly placed in shaking uncubator with temperature setpoint of 23 deg C at 100 rpm
- Initial cells density: 1.0 x 10^4 cells/mL
- Control end cells density: average 164.5 x 10^4 cells/mL

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Sterile test conditions: yes, Erlenmeyers were sterilized
- Adjustment of pH: no
- Photoperiod: continuous
- Light intensity and quality: 400-700 nm, 78 µE/m².s

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: counting chamber, Neubauer improved and microscope (magnification 200 times)

TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: based on the results of a preliminary test, the final study was conducted as a limit test at 100 mg/l.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

< 100 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

- Exponential growth in the control (for algal test): yes
- Effect concentrations exceeding solubility of substance in test medium: test substance completely miscible

Results with reference substance (positive control):

- Results with reference substance valid? yes
- EC50(72 h) for biomass integral 0.55 mg/L and for growth rate 1.1 mg/L

Reported statistics and error estimates:

NOEC based on biomass integral at test termination was determined with Williams' test.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In the current limit test with Selenastrum capricornutum at a test substance concentration of 100 mg/L , the ErC50 for algae growth rate was >100 mg/L. The NOEC was <100 mg/L.