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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
6 - 9 December 2004
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: The study has been performed according to the OECD guideline and according to GLP principles.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2005
Report date:
2005

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
3-chloropropane-1,2-diol
EC Number:
202-492-4
EC Name:
3-chloropropane-1,2-diol
Cas Number:
96-24-2
Molecular formula:
C3H7ClO2
IUPAC Name:
3-chloropropane-1,2-diol
Details on test material:
- Name of test material (as cited in study report): 3-chloro-1,2-propanediol
- Synonym: alphaMCG, glycerol-alpha-monochlorohydrine
- Substance type: light yellow to almost colourless viscous liquid
- Physical state: liquid
- Analytical purity: 99.3 % (GC)
- Purity test date: no data, re-test date February 2005
- Lot/batch No.: A013405101
- Storage condition of test material: room temperature
- Other: supplier: Acros Organics, 's-Hertogenbosch, The Netherlands

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: algal medium (0 mg/L), 100 mg/L nominal
- Sampling method: triplicate aliquots of 100 µL of stock solution and algal medium on day 0 and 1.0 mL aliquots of all test solutions on day 3
- Sample storage conditions before analysis: samples (from day 3) were centrifuged to remove algae. Aliquots of day 0 and 100 ul of the supernatant of day 3 samples were transferred into a HPLC vial, containing 900 ul of trichloropropane (5 mg/L) in methanol and stored at 2-8 deg C, maximum 5 days

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
* preparation of stock solution: 110 mg of 3-chloro-1,2-propanediol (alphaMCG) in 1 liter algal medium resulting in a concentration of 0.11 g/L
* test solution: 90 mL of stock solution and 10 mL of algal medium resulting in a concentration of 100 mg/L
- Controls: yes

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Common name: freshwater green alga
- Strain: ATCC 22662
- Source (laboratory, culture collection): Culture collection of algae and protozoa, Ambleside, Cumbria, UK
- Method of cultivation: 100-mL Erlenmeyer flasks with 50 mL filter sterilised medium were used for algae culture. Each week 0.5-2 ml of the culture is transferred to fresh algal medium. The preculture used for the study was 4 days of age at the start of the test.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h
Post exposure observation period:
not applicable

Test conditions

Hardness:
no data
Test temperature:
23.8-24.3 deg C
pH:
7.7-8.9
Dissolved oxygen:
no data
Salinity:
not applicable
Nominal and measured concentrations:
nominal concentrations: 0 mg/L and 100 mg/L
measured concentrations: below detection limit and 97.4-105 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 100-mL Erlenmeyer flasks randomly placed in shaking uncubator with temperature setpoint of 23 deg C at 100 rpm
- Initial cells density: 1.0 x 10^4 cells/mL
- Control end cells density: average 164.5 x 10^4 cells/mL

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Sterile test conditions: yes, Erlenmeyers were sterilized
- Adjustment of pH: no
- Photoperiod: continuous
- Light intensity and quality: 400-700 nm, 78 µE/m².s

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: counting chamber, Neubauer improved and microscope (magnification 200 times)

TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: based on the results of a preliminary test, the final study was conducted as a limit test at 100 mg/l.
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
< 100 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
growth rate
Details on results:
- Exponential growth in the control (for algal test): yes
- Effect concentrations exceeding solubility of substance in test medium: test substance completely miscible
Results with reference substance (positive control):
- Results with reference substance valid? yes
- EC50(72 h) for biomass integral 0.55 mg/L and for growth rate 1.1 mg/L
Reported statistics and error estimates:
NOEC based on biomass integral at test termination was determined with Williams' test.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In the current limit test with Selenastrum capricornutum at a test substance concentration of 100 mg/L , the ErC50 for algae growth rate was >100 mg/L. The NOEC was <100 mg/L.

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