3-chloropropane-1,2-diol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14-16 December 2004

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study has been performed according to the OECD guideline and according to GLP principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2005

Report date:

2005

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-chloropropane-1,2-diol

EC Number:

202-492-4

EC Name:

3-chloropropane-1,2-diol

Cas Number:

96-24-2

Molecular formula:

C3H7ClO2

IUPAC Name:

3-chloropropane-1,2-diol

Details on test material:

- Name of test material (as cited in study report): 3-chloro-1,2-propanediol
- Synonym: alphaMCG, glycerol-alpha-monochlorohydrine
- Substance type: light yellow to almost colourless viscous liquid
- Physical state: liquid
- Analytical purity: 99.3 % (GC)
- Purity test date: no data, re-test date February 2005
- Lot/batch No.: A013405101
- Storage condition of test material: room temperature
- Other: supplier: Acros Organics, 's-Hertogenbosch, The Netherlands

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: nominal 0 mg/L and 100 mg/L
- Sampling method: day 0: triplicate samples of 100 µL from stock solution and Elendt M4 medium, day 2: samples of 100 µL taken from all test solutions
- Sample storage conditions before analysis: samples were transferred into a HPLC vial containing 900 ul methanol (with 5 mg/l trichloropropane as internal standard) and stored at 2-8 deg C for maximum 3 days

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
* preparation of stock solution: 151 mg of 3-chloro-1,2-propanediol dissolved in 1.5 L Elendt M4 medium
* test solution: 200 mL of stock solution
- Controls: 200 mL of Elendt M4 medium

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water fleas
- Strain: no data
- Source: Aquasense b.v., Amsterdam, The Netherlands
- Age at study initiation (mean and range, SD): 0-24 h
- Method of breeding: cultured in 2-L vessels with Elandt M4 medium
- Feeding during test
- Food type: algal suspension of Chlorella vulgaris

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

not applicable

Test conditions

Hardness:

no data

Test temperature:

20.6 deg C

pH:

8.0-8.1

Dissolved oxygen:

8.8 mg/L

Salinity:

not applicable

Nominal and measured concentrations:

nominal concentrations: 0 mg/L and 100 mg/L
measured concentrations: below detection limit and 107-112 mg/L (day 0-day 2)(mean: 109 mg/L)

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 2-L vessels
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: purified water prepared according to the methods of the European Pharmacopee and the USP Purified Water

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility

TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: based on the results of a preliminary study (no data) a limit test was conducted.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

No adverse effects observed

Results with reference substance (positive control):

- Results with reference substance valid? yes
- EC50(24 h): 1.01 mg/L with a 95% CL of 0.94-1.07 mg/L.

Reported statistics and error estimates:

NOEC for immobility was determined with Fishers's exact test.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In the current limit test at a nominal concentration of 100 mg/L, the test substance did not induce immobility in daphnia magna.