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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Not reported
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: This study was not conducted in a GLP facility, but was conducted in accordance with the DIN 38412, Part II (Daphnia short-time test) (German Institute of Standardization, 1982).

Data source

Reference
Reference Type:
publication
Title:
Results of the harmful effects of selected water pollutants (anilines, phenols, aliphatic compounds) to Daphnia Magna
Author:
R Kuhn et al.
Year:
1989
Bibliographic source:
Renate Kuhn, Monika Pattard, Klaus-Dieter Pernak and Angeal Winter (1989) Results of the harmful effects of selected water pollutants (anilines, phenols, aliphatic compounds) to Daphnia Magna. Water Research, 23(4): 495-499.

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: DIN 38412, Part II (Daphnia short-time test) (German Institute of Standardization, 1982)
Deviations:
not specified
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna from an in-house culture between 6-24 hours old were used for the study.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.4 mmol/L
Test temperature:
20 degrees celsius
pH:
8.0 +/- 0.2
Dissolved oxygen:
The dissolved oxygen content did not fall below 2 mg/L for the duration of the study.
Nominal and measured concentrations:
The actual test concentrations were not reported in the publication, but the following information was provided:
"The concentration steps of the test solution were selected so as to give 3-2 EC values in a range between EC0 and EC100, of which at least one value was below and one above EC50.
Details on test conditions:
The test vessels were two 50 mL beakers containing 20 mL solution per concentration for both the test concentrations and the control. Ten daphnia were added to each vessel.
After a test period of 24 h and 48 h, the number of animals in the control and test solutions that were not immobile were counted. After 48 hours the pH was also measured to determine whether the validity criterion was achieved. The dissolved oxygen concentration was also determined at the end of the study to ensure that the validity criterion was achieved.
The test was considered valid when fewer thatn 10 % of control animals were immobile at the end of the study, when the pH remained above 7.0 and the dissolved oxygen concentration did not fall below 2 mg/L at the end of the study.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
6.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL: 2.6-17
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
2.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL: 2.0-3.7

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 48 hour EC50 of the test item to Daphnia magna is 2.7 mg/L (95% CL: 2.0-3.7).