Ammonium perrhenate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Not reported

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP, non-guideline, experimental study, no restrictions, fully adequate for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

A single acute oral administration of ammonium perrhenate was given to rats in a range-finding study at doses of up to 2000 mg/kg bw. A further (main) study was planned using the highest dose level causing no deaths in the range-finding study, however further samples of the test material were unavailable.

GLP compliance:

no

Remarks:

Conducted prior to requirement for GLP

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Ammonium perrhenate

EC Number:

237-075-6

EC Name:

Ammonium perrhenate

Cas Number:

13598-65-7

Molecular formula:

H4N.O4Re

IUPAC Name:

Ammonium oxido(trioxo)rhenium

Details on test material:

- Name of test material (as cited in study report): ammonium perrhenate
- Substance type: white crystalline solid

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Charles River (UK) Ltd, Margate, Kent
- Age at study initiation: “young adult”
- Weight at study initiation: 175-240 g
- Fasting period before study: overnight fast
- Housing: 5 rats of one sex in polypropylene cages
- Diet: ad libitum oxoid maintenance diet supplied by Herbert C. Styles (Bewdley) Ltd
- Water: ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): “thermostatically controlled room”
- Photoperiod (hrs dark / hrs light): “controlled lighting conditions”

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

vegetable oil

Details on oral exposure:

The dose was administered by a single peroral injection using a metal cannula. The test material was dissolved in vegetable oil on a weight/volume basis to contain the required dose per kg bw in 10 ml of the vegetable oil.

Doses:

Male and female rats were administered 25, 50, 200, 500 or 2000 mg/kg bw.

No. of animals per sex per dose:

One rat/sex/dose

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: immediately after dosing, 4 hrs after dosing, and daily for 14 days.
- Necropsy of survivors performed: no
- Other examinations performed: clinical signs

Statistics:

None

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Mortality:

No deaths were reported within the 14 day observation period.

Clinical signs:

other: No overt signs of toxicity were reported within the 14 day observation period.

Gross pathology:

Not reported

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The acute oral median lethal dose (LD50) of ammonium perrhenate in the rat was found, in a range-finding study, to be >2000 mg/kg bw.

Executive summary:

In a range-finding study, one male and one female rat/group were administered 25, 50, 200, 500 or 2000 mg ammonium perrhenate/kg bw by stomach tube. No deaths were seen in either sex at any dose level. A further study was intended using groups of 5 male and 5 female rats and treating them at the highest dose level causing no deaths in the range-finding study (i.e. 2000 mg/kg bw). However, due to the unavailability of the test material, this was not possible.

 

The study authors concluded that “subject to main study confirmation, the results suggest that the LD50 …. may be in excess of 2000 mg/kg body weight”, and classed the test substance according to Hodge and Sterner (1943) as “slightly toxic”.