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EC number: 246-625-4 | CAS number: 25111-05-1
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
No effects up to the limit of water solubility (OECD 201), read across.
Key value for chemical safety assessment
Additional information
Since no studies investigating the toxicity of 2-ethyl-2-(hydroxymethyl)-1,3-propanediyl dioleate (CAS 25111-05-1) to aquatic algae are available, in accordance to Regulation (EC) No 1907/2006 Annex XI, 1.5 a read-across to the structurally related source substances Fatty acids, C16-18 even numbered and C18-unsatd. triesters with propylidynetrimethanol (former CAS 57675-44-2) and Fatty acids, C8-10 (even), C14-18 (even) and C16-18 (even)-unsatd., triesters with trimethylolpropane (former CAS 85186-89-6) was conducted.
The target substance is characterized by C18 unsaturated fatty acid esterified trimethylolpropane (TMP) (mainly di- and triester). The selected source substances are structurally very similar to the target substance and are thus adequate for the assessment of short-term toxicity to aquatic invertebrates. Fatty acids, C16-18 even numbered and C18-unsatd. triesters with propylidynetrimethanol (former CAS 57675-44-2) is characterized by C18, C18 unsatd fatty acids triesterified with TMP. Fatty acids, C8-10 (even), C14-18 (even) and C16-18 (even)-unsatd., triesters with trimethylolpropane (former CAS 85186-89-6) mainly consists of C18:1 FA triesterified with TMP. This read-across is justified in detail in the overall summary (IUCLID Section 6.1) and within the analogue justification in IUCLID Section 13. In this case of read-across, the best suited (highest degree of structural similarity, nearest physico-chemical properties) read-across substance was used for the assessment.
Two studies, investigating the toxicity to freshwater algae of the source substance fatty acids, C16-18 even numbered and C18-unsatd. triesters with propylidynetrimethanol (former CAS 57675-44-2) are available. During both static Algal Growth Inhibition tests low inhibiting effects on growth rate and biomass were observed within 72 hour test period at the tested concentrations. The first study according to OECD 201 with Desmodesmus subspicatus determined an ErL0 (72 h) > 100 mg/L (WAF loading rate) based on growth rate (Häner, 2006). In the second study performed similar to OECD 201 with Pseudokirchneriella subcapitata a low inhibition of growth was observed (11%) at the WAF loading rate of 1000 mg/L after 72 h (ErL20 (72 h) > 1000 mg/L) (Craig, 2005).
Four studies, investigating the toxicity to freshwater algae, and one study, investigating the toxicity to marine algae, of the source substance Fatty acids, C8-10 (even), C14-18 (even) and C16-18 (even)-unsatd., triesters with trimethylolpropane (former CAS 85186-89-6), are available. The first study was performed according to EU Method C.3 (GLP) with the freshwater algae Desmodesmus subspicatus (Wierich and Özkabakcioglu, 1999). The study resulted in an EL50 (72 h) > 100 mg/L (WAF loading rate) based on the growth rate. Both supporting studies with Pseudokirchneriella subcapitata according to OECD 201 (GLP) showed significant inhibition of the growth rate (58% and 67%) at the highest test concentration (1000 mg/L WAF loading rate) (Craig, 2005). Because of the serial dilution from the highest concentration (1000 mg/L) in both studies, only the 1000 mg/L test concentration was valid and could be used for evaluation of toxicity of the test substance. Therefore, the ErL58 and ErL67 were determined as 1000 mg/L. The marine study was performed with Skeletonema costatum according to ISO 10253 (GLP). The EL50 (72 h) was calculated as 9865.86 mg/L and the NOELR (72 h) as 3200 mg/L. Overall, the data show adverse effects of Fatty acids, C8-10 (even), C14-18 (even) and C16-18 (even)-unsatd., triesters with trimethylolpropane (former CAS 85186-89-6) only far in excess of natural occurring concentrations. Therefore, the source substance is not harmful to freshwater and marine algae.
Based on the available result from several structurally related read-across substances (in accordance to Regulation (EC) No 1907/2006 Annex XI, 1.5) which are characterized by a similar ecotoxicological profile and comparable structure, it can be concluded that2-ethyl-2-(hydroxymethyl)-1,3-propanediyl dioleatewill not exhibit effects to aquatic algae up to the limit of water solubility.
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