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Long-term toxicity to aquatic invertebrates

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Reference
Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Specific details on test material used for the study:
- Name of test material (as cited in study report): C-SAT 070029
- Physical state: pale yellow-amber, viscous liquid
- Analytical purity: > 89%
- Solvent: < 2% Toluene
- Lot/batch No.: JB 620-70-1
- Expiration date of the lot/batch: 11.10.2011
- Storage condition of test material: room temp., dark
Analytical monitoring:
yes
Details on sampling:
- Concentrations: All test concentrations and control groups were analytically verified via HPLC in the fresh media (0 h} on day O, 7, 14, 19 and old media (4 h) on days 2, 9, 16, 21. For old media replicates were prepared without test organisms and stored under test conditions.
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: T he stock solution (1 mg/L was weighed out) was prepared with dilution water one day prior to application. The stock solution was stirred with approx. 1100 rpm for 24 h at room temperature. The test concentrations of 0.1 to 0.32 mg/L were achieved by serial dilution of the stock solution.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name:water flea
- Source: Institut für Wasser-, Boden- und Lufthygiene, Berlin
- Age of parental stock (mean and range, SD):
- Feeding during test
- Food type: green algae (> 10E6 cells/mL)
- Amount: ad libitum
- Frequency: at least 5 times per week
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d
Hardness:
140 mg/L as CaCO3 (mean)
Test temperature:
19.9 - 21.7 °C
pH:
7.73 - 8.62
Dissolved oxygen:
8.11 - 8.33 mg/L
Nominal and measured concentrations:
Nominal: 0.01, 0.032, 0.1, 0.32 and 1.0 mg/L
Measured: 0.00952, 0.0267, 0.189, 0.308 and 0.986 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 100 mL glass beakers with 50 mL test solution
- Renewal rate of test solution (frequency/flow rate): 3 times per week
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4
- Culture medium different from test medium: same
- Intervals of water quality measurement: measured once a week in fresh and old media in one replicate of the control and the highest concentration of the test item

EFFECT PARAMETERS MEASURED: Mortality of parent daphnids, first appearance of neonates, number og aborted eggs and abnormalities were observed daily. Length and body weight was measured at the end of the test.
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Key result
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.189 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
0.308 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Mortality of parent animals: 30% at 0.308 mg/L
- No. of offspring produced per day per female: 47.2 - 56.3 for the concentration up to 0.308 mg/L (control: 60.8)
- Time to first brood release or time to hatch: 8 - 8.6 for control and concentration up to 0.189 mg/L (0.308 mg/L: 10.4)
Validity criteria fulfilled:
yes
Conclusions:
In a GLP study according to OECD Test Guideline 211 (21-day Daphnia magna reproduction test), the NOEC for acute toxicity of the test substance was determined to be 0.189 mg/L.
Executive summary:

The chronic toxicity of the test substance to daphnids was evaluated in a study according to OECD Test Gudieline 211 (Daphnia magna reproduction test) under GLP conditions. In a semi-static study Daphnia magna were exposed for 21 days. The test material was applied directly to the test vessels. Analytical monitoring was performed. All validity criteria were fulfilled. The NOEC based on reproduction (increased time to first brood release or time to hatch) is determined to be 0.189 mg/L based on measured concentrations.

Description of key information

NOEC (21d) = 0.189 mg/L (measured) for Daphnia magna (OECD 211)

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater invertebrates:
0.189 mg/L

Additional information

One study investigating the long-term toxicity of the test substance to aquatic invertebrates is available. The study was performed according to OECD guideline 211 under GLP conditions (Noack 2008). The test material was applied directly to the test vessels. Analytical monitoring was performed. All validity criteria were fulfilled. The NOEC based on reproduction (increased time to first brood release or time to hatch) is determined to be 0.189 mg/L based on measured concentration.